LSBio
Human CAGE1 / Cancer Antigen 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CAGE1 / Cancer Antigen 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CAGE1 antibodies →
$220.00each
Catalog Number: LS-G93822-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CAGE1 (NM_205864) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA69L16.7 | Cancer/testis antigen 95 | CT3 | CAGE-1 | Cancer/testis antigen 3 | Cancer/testis antigen gene 1 | CT95 | CTAG3 | CAGE1 | Cancer antigen 1
Origin Species
Human
SwissProt
Q8TC20

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTEKPEFQSQ VYNYAKDNNI KQDSFKEENP METSVSANTD QLGNEYFRQP PPRSPPLIHC
61SGEMLKFTEK SLAKSIAKES ALNPSQPPSF LCKTAVPSKE IQNYGEIPEM SVSYEKEVTA
121EGVERPEIVS TWSSAGISWR SEACRENCEM PDWEQSAESL QPVQEDMALN EVLQKLKHTN
181RKQEVRIQEL QCSNLYLEKR VKELQMKITK QQVFIDVINK LKENVEELIE DKYKIILEKN
241DTKKTLQNLE EVLANTQKHL QESRNDKEML QLQFKKIKAN YVCLQERYMT EMQQKNKSVS
301QYLEMDKTLS KKEEEVERLQ QLKKELEKAT ASALDLLKRE KEAQEQEFLS LQEEFQKLEK
361ENLEERQKLK SRLEKLLTQV RNLQFMSENE RTKNIKLQQQ INEVKNENAK LKQQVARSEE
421QNYVPKFETA QLKDQLEEVL KSDITKDTKT THSNLLPDCS PCEERLNPAD IKRASQLASK
481MHSLLALMVG LLTCQDIINS DAEHFKESEK VSDIMLQKLK SLHLKKKTLD KEVIDCDSDE
541AKSIRDVPTL LGAKLDKYHS LNEELDFLVT SYEEIIECAD QRLAISHSQI AHLEERNKHL
601EDLIRKPREK ARKPRSKSLE NHPKSMTMMP ALFKENRNDL D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

641 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.