Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAGE1 / Cancer Antigen 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102993-100
Catalog Number: LS-G102993
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CAGE1 (NM_205864), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA69L16.7 | Cancer/testis antigen 95 | CT3 | CAGE-1 | Cancer/testis antigen 3 | Cancer/testis antigen gene 1 | CT95 | CTAG3 | CAGE1 | Cancer antigen 1
- Origin Species
- Human
- SwissProt
- Q8TC20
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTEKPEFQSQ VYNYAKDNNI KQDSFKEENP METSVSANTD QLGNEYFRQP PPRSPPLIHC61SGEMLKFTEK SLAKSIAKES ALNPSQPPSF LCKTAVPSKE IQNYGEIPEM SVSYEKEVTA121EGVERPEIVS TWSSAGISWR SEACRENCEM PDWEQSAESL QPVQEDMALN EVLQKLKHTN181RKQEVRIQEL QCSNLYLEKR VKELQMKITK QQVFIDVINK LKENVEELIE DKYKIILEKN241DTKKTLQNLE EVLANTQKHL QESRNDKEML QLQFKKIKAN YVCLQERYMT EMQQKNKSVS301QYLEMDKTLS KKEEEVERLQ QLKKELEKAT ASALDLLKRE KEAQEQEFLS LQEEFQKLEK361ENLEERQKLK SRLEKLLTQV RNLQFMSENE RTKNIKLQQQ INEVKNENAK LKQQVARSEE421QNYVPKFETA QLKDQLEEVL KSDITKDTKT THSNLLPDCS PCEERLNPAD IKRASQLASK481MHSLLALMVG LLTCQDIINS DAEHFKESEK VSDIMLQKLK SLHLKKKTLD KEVIDCDSDE541AKSIRDVPTL LGAKLDKYHS LNEELDFLVT SYEEIIECAD QRLAISHSQI AHLEERNKHL601EDLIRKPREK ARKPRSKSLE NHPKSMTMMP ALFKENRNDL DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
641 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.