LSBio
Human CACNA1I / Cav3.3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CACNA1I / Cav3.3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CACNA1I antibodies →
$220.00each
Catalog Number: LS-G91479-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CACNA1I (NM_021096) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Alpha 1i | CACNA1I | KIAA1120 | Ca(v)3.3 | Cav3.3
Origin Species
Human
NCBI GenBank
NM_021096
SwissProt
Q9P0X4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
244.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAESASPPSS SAAAPAAEPG VTTEQPGPRS PPSSPPGLEE PLDGADPHVP HPDLAPIAFF
61CLRQTTSPRN WCIKMVCNPW FECVSMLVIL LNCVTLGMYQ PCDDMDCLSD RCKILQVFDD
121FIFIFFAMEM VLKMVALGIF GKKCYLGDTW NRLDFFIVMA GMVEYSLDLQ NINLSAIRTV
181RVLRPLKAIN RVPSMRILVN LLLDTLPMLG NVLLLCFFVF FIFGIIGVQL WAGLLRNRCF
241LEENFTIQGD VALPPYYQPE EDDEMPFICS LSGDNGIMGC HEIPPLKEQG RECCLSKDDV
301YDFGAGRQDL NASGLCVNWN RYYNVCRTGS ANPHKGAINF DNIGYAWIVI FQVITLEGWV
361EIMYYVMDAH SFYNFIYFIL LIIVGSFFMI NLCLVVIATQ FSETKQREHR LMLEQRQRYL
421SSSTVASYAE PGDCYEEIFQ YVCHILRKAK RRALGLYQAL QSRRQALGPE APAPAKPGPH
481AKEPRHYHGK TKGQGDEGRH LGSRHCQTLH GPASPGNDHS GRELCPQHSP LDATPHTLVQ
541PIPATLASDP ASCPCCQHED GRRPSGLGST DSGQEGSGSG SSAGGEDEAD GDGARSSEDG
601ASSELGKEEE EEEQADGAVW LCGDVWRETR AKLRGIVDSK YFNRGIMMAI LVNTVSMGIE
661HHEQPEELTN ILEICNVVFT SMFALEMILK LAAFGLFDYL RNPYNIFDSI IVIISIWEIV
721GQADGGLSVL RTFRLLRVLK LVRFMPALRR QLVVLMKTMD NVATFCMLLM LFIFIFSILG
781MHIFGCKFSL RTDTGDTVPD RKNFDSLLWA IVTVFQILTQ EDWNVVLYNG MASTSPWASL
841YFVALMTFGN YVLFNLLVAI LVEGFQAEGD ANRSYSDEDQ SSSNIEEFDK LQEGLDSSGD
901PKLCPIPMTP NGHLDPSLPL GGHLGPAGAA GPAPRLSLQP DPMLVALGSR KSSVMSLGRM
961SYDQRSLSSS RSSYYGPWGR SAAWASRRSS WNSLKHKPPS AEHESLLSAE RGGGARVCEV
1021AADEGPPRAA PLHTPHAHHI HHGPHLAHRH RHHRRTLSLD NRDSVDLAEL VPAVGAHPRA
1081AWRAAGPAPG HEDCNGRMPS IAKDVFTKMG DRGDRGEDEE EIDYTLCFRV RKMIDVYKPD
1141WCEVREDWSV YLFSPENRFR VLCQTIIAHK LFDYVVLAFI FLNCITIALE RPQIEAGSTE
1201RIFLTVSNYI FTAIFVGEMT LKVVSLGLYF GEQAYLRSSW NVLDGFLVFV SIIDIVVSLA
1261SAGGAKILGV LRVLRLLRTL RPLRVISRAP GLKLVVETLI SSLKPIGNIV LICCAFFIIF
1321GILGVQLFKG KFYHCLGVDT RNITNRSDCM AANYRWVHHK YNFDNLGQAL MSLFVLASKD
1381GWVNIMYNGL DAVAVDQQPV TNHNPWMLLY FISFLLIVSF FVLNMFVGVV VENFHKCRQH
1441QEAEEARRRE EKRLRRLEKK RRKAQRLPYY ATYCHTRLLI HSMCTSHYLD IFITFIICLN
1501VVTMSLEHYN QPTSLETALK YCNYMFTTVF VLEAVLKLVA FGLRRFFKDR WNQLDLAIVL
1561LSVMGITLEE IEINAALPIN PTIIRIMRVL RIARVLKLLK MATGMRALLD TVVQALPQVG
1621NLGLLFMLLF FIYAALGVEL FGKLVCNDEN PCEGMSRHAT FENFGMAFLT LFQVSTGDNW
1681NGIMKDTLRD CTHDERSCLS SLQFVSPLYF VSFVLTAQFV LINVVVAVLM KHLDDSNKEA
1741QEDAEMDAEL ELEMAHGLGP GPRLPTGSPG APGRGPGGAG GGGDTEGGLC RRCYSPAQEN
1801LWLDSVSLII KDSLEGELTI IDNLSGSIFH HYSSPAGCKK CHHDKQEVQL AETEAFSLNS
1861DRSSSILLGD DLSLEDPTAC PPGRKDSKGE LDPPEPMRVG DLGECFFPLS STAVSPDPEN
1921FLCEMEEIPF NPVRSWLKHD SSQAPPSPFS PDASSPLLPM PAEFFHPAVS ASQKGPEKGT
1981GTGTLPKIAL QGSWASLRSP RVNCTLLRQA TGSDTSLDAS PSSSAGSLQT TLEDSLTLSD
2041SPRRALGPPA PAPGPRAGLS PAARRRLSLR GRGLFSLRGL RAHQRSHSSG GSTSPGCTHH
2101DSMDPSDEEG RGGAGGGGAG SEHSETLSSL SLTSLFCPPP PPPAPGLTPA RKFSSTSSLA
2161APGRPHAAAL AHGLARSPSW AADRSKDPPG RAPLPMGLGP LAPPPQPLPG ELEPGDAASK
2221RKR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2223 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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