Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CACNA1I / Cav3.3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110773-100
Catalog Number: LS-G110773
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CACNA1I (NM_021096), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha 1i | CACNA1I | KIAA1120 | Ca(v)3.3 | Cav3.3
- Origin Species
- Human
- NCBI GenBank
- NM_021096
- SwissProt
- Q9P0X4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 244.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAESASPPSS SAAAPAAEPG VTTEQPGPRS PPSSPPGLEE PLDGADPHVP HPDLAPIAFF61CLRQTTSPRN WCIKMVCNPW FECVSMLVIL LNCVTLGMYQ PCDDMDCLSD RCKILQVFDD121FIFIFFAMEM VLKMVALGIF GKKCYLGDTW NRLDFFIVMA GMVEYSLDLQ NINLSAIRTV181RVLRPLKAIN RVPSMRILVN LLLDTLPMLG NVLLLCFFVF FIFGIIGVQL WAGLLRNRCF241LEENFTIQGD VALPPYYQPE EDDEMPFICS LSGDNGIMGC HEIPPLKEQG RECCLSKDDV301YDFGAGRQDL NASGLCVNWN RYYNVCRTGS ANPHKGAINF DNIGYAWIVI FQVITLEGWV361EIMYYVMDAH SFYNFIYFIL LIIVGSFFMI NLCLVVIATQ FSETKQREHR LMLEQRQRYL421SSSTVASYAE PGDCYEEIFQ YVCHILRKAK RRALGLYQAL QSRRQALGPE APAPAKPGPH481AKEPRHYHGK TKGQGDEGRH LGSRHCQTLH GPASPGNDHS GRELCPQHSP LDATPHTLVQ541PIPATLASDP ASCPCCQHED GRRPSGLGST DSGQEGSGSG SSAGGEDEAD GDGARSSEDG601ASSELGKEEE EEEQADGAVW LCGDVWRETR AKLRGIVDSK YFNRGIMMAI LVNTVSMGIE661HHEQPEELTN ILEICNVVFT SMFALEMILK LAAFGLFDYL RNPYNIFDSI IVIISIWEIV721GQADGGLSVL RTFRLLRVLK LVRFMPALRR QLVVLMKTMD NVATFCMLLM LFIFIFSILG781MHIFGCKFSL RTDTGDTVPD RKNFDSLLWA IVTVFQILTQ EDWNVVLYNG MASTSPWASL841YFVALMTFGN YVLFNLLVAI LVEGFQAEGD ANRSYSDEDQ SSSNIEEFDK LQEGLDSSGD901PKLCPIPMTP NGHLDPSLPL GGHLGPAGAA GPAPRLSLQP DPMLVALGSR KSSVMSLGRM961SYDQRSLSSS RSSYYGPWGR SAAWASRRSS WNSLKHKPPS AEHESLLSAE RGGGARVCEV1021AADEGPPRAA PLHTPHAHHI HHGPHLAHRH RHHRRTLSLD NRDSVDLAEL VPAVGAHPRA1081AWRAAGPAPG HEDCNGRMPS IAKDVFTKMG DRGDRGEDEE EIDYTLCFRV RKMIDVYKPD1141WCEVREDWSV YLFSPENRFR VLCQTIIAHK LFDYVVLAFI FLNCITIALE RPQIEAGSTE1201RIFLTVSNYI FTAIFVGEMT LKVVSLGLYF GEQAYLRSSW NVLDGFLVFV SIIDIVVSLA1261SAGGAKILGV LRVLRLLRTL RPLRVISRAP GLKLVVETLI SSLKPIGNIV LICCAFFIIF1321GILGVQLFKG KFYHCLGVDT RNITNRSDCM AANYRWVHHK YNFDNLGQAL MSLFVLASKD1381GWVNIMYNGL DAVAVDQQPV TNHNPWMLLY FISFLLIVSF FVLNMFVGVV VENFHKCRQH1441QEAEEARRRE EKRLRRLEKK RRKAQRLPYY ATYCHTRLLI HSMCTSHYLD IFITFIICLN1501VVTMSLEHYN QPTSLETALK YCNYMFTTVF VLEAVLKLVA FGLRRFFKDR WNQLDLAIVL1561LSVMGITLEE IEINAALPIN PTIIRIMRVL RIARVLKLLK MATGMRALLD TVVQALPQVG1621NLGLLFMLLF FIYAALGVEL FGKLVCNDEN PCEGMSRHAT FENFGMAFLT LFQVSTGDNW1681NGIMKDTLRD CTHDERSCLS SLQFVSPLYF VSFVLTAQFV LINVVVAVLM KHLDDSNKEA1741QEDAEMDAEL ELEMAHGLGP GPRLPTGSPG APGRGPGGAG GGGDTEGGLC RRCYSPAQEN1801LWLDSVSLII KDSLEGELTI IDNLSGSIFH HYSSPAGCKK CHHDKQEVQL AETEAFSLNS1861DRSSSILLGD DLSLEDPTAC PPGRKDSKGE LDPPEPMRVG DLGECFFPLS STAVSPDPEN1921FLCEMEEIPF NPVRSWLKHD SSQAPPSPFS PDASSPLLPM PAEFFHPAVS ASQKGPEKGT1981GTGTLPKIAL QGSWASLRSP RVNCTLLRQA TGSDTSLDAS PSSSAGSLQT TLEDSLTLSD2041SPRRALGPPA PAPGPRAGLS PAARRRLSLR GRGLFSLRGL RAHQRSHSSG GSTSPGCTHH2101DSMDPSDEEG RGGAGGGGAG SEHSETLSSL SLTSLFCPPP PPPAPGLTPA RKFSSTSSLA2161APGRPHAAAL AHGLARSPSW AADRSKDPPG RAPLPMGLGP LAPPPQPLPG ELEPGDAASK2221RKRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
2223 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.