C14orf159 Antibody
Catalog Number: LS-C830564
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human C14orf159 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G82245-20
Catalog Number: LS-G82245
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C14orf159 (NM_024952) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C14orf159
- Origin Species
- Human
- NCBI GenBank
- NM_024952
- SwissProt
- Q7Z3D6
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPFTLHLRSR LPSAIRSLIL QKKPNIRNTS SMAGELRPAS LVVLPRSLAP AFERFCQVNT61GPLPLLGQSE PEKWMLPPQG AISETRMGHP QFWKYEFGAC TGSLASLEQY SEQLKDMVAF121FLGCSFSLEE ALEKAGLPRR DPAGHSQTTV PCVTHAGFCC PLVVTMRPIP KDKLEGLVRA181CCSLGGEQGQ PVHMGDPELL GIKELSKPAY GDAMVCPPGE VPVFWPSPLT SLGAVSSCET241PLAFASIPGC TVMTDLKDAK APPGCLTPER IPEVHHISQD PLHYSIASVS ASQKIRELES301MIGIDPGNRG IGHLLCKDEL LKASLSLSHA RSVLITTGFP THFNHEPPEE TDGPPGAVAL361VAFLQALEKE VAIIVDQRAW NLHQKIVEDA VEQGVLKTQI PILTYQGGSV EAAQAFLCKN421GDPQTPRFDH LVAIERAGRA ADGNYYNARK MNIKHLVDPI DDLFLAAKKI PGISSTGVGD481GGNELGMGKV KEAVRRHIRH GDVIACDVEA DFAVIAGVSN WGGYALACAL YILYSCAVHS541QYLRKAVGPS RAPGDQAWTQ ALPSVIKEEK MLGILVQHKV RSGVSGIVGM EVDGLPFHNT601HAEMIQKLVD VTTAQVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
616 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
