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C14orf159 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C14orf159 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C14orf159 antibodies →
$725.00each
Catalog Number: LS-G118786-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C14orf159 (NM_001102367), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf159
Origin Species
Human
NCBI GenBank
NM_024952
SwissProt
Q7Z3D6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPFTLHLRSR LPSAIRSLIL QKKPNIRNTS SMAGELRPAS LVVLPRSLAP AFERFCQVNT
61GPLPLLGQSE PEKWMLPPQG AISETRMGHP QFWKYEFGAC TGSLASLEQY SEQLKDMVAF
121FLGCSFSLEE ALEKAGLPRR DPAGHSQTTV PCVTHAGFCC PLVVTMRPIP KDKLEGLVRA
181CCSLGGEQGQ PVHMGDPELL GIKELSKPAY GDAMVCPPGE VPVFWPSPLT SLGAVSSCET
241PLAFASIPGC TVMTDLKDAK APPGCLTPER IPEVHHISQD PLHYSIASVS ASQKIRELES
301MIGIDPGNRG IGHLLCKDEL LKASLSLSHA RSVLITTGFP THFNHEPPEE TDGPPGAVAL
361VAFLQALEKE VAIIVDQRAW NLHQKIVEDA VEQGVLKTQI PILTYQGGSV EAAQAFLCKN
421GDPQTPRFDH LVAIERAGRA ADGNYYNARK MNIKHLVDPI DDLFLAAKKI PGISSTGVGD
481GGNELGMGKV KEAVRRHIRH GDVIACDVEA DFAVIAGVSN WGGYALACAL YILYSCAVHS
541QYLRKAVGPS RAPGDQAWTQ ALPSVIKEEK MLGILVQHKV RSGVSGIVGM EVDGLPFHNT
601HAEMIQKLVD VTTAQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

616 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.