BRN3B / POU4F2 Antibody (aa311-339)
Catalog Number: LS-C163879-400
400 µL / $401.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human BRN3B / POU4F2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G88058-20
Catalog Number: LS-G88058
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human POU4F2(POU4F2) (NM_004575) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brn-3b | BRN3.2 | POU class 4 homeobox 2 | POU4F2 | POU domain protein | Brain-3B | BRN3B
- Origin Species
- Human
- NCBI GenBank
- NM_004575
- SwissProt
- Q12837
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MMMMSLNSKQ AFSMPHGGSL HVEPKYSALH STSPGSSAPI APSASSPSSS SNAGGGGGGG61GGGGGGGGGR SSSSSSSGSS GGGGSEAMRR ACLPTPPSNI FGGLDESLLA RAEALAAVDI121VSQSKSHHHH PPHHSPFKPD ATYHTMNTIP CTSAASSSSV PISHPCALAG THHHHHHHHH181HHHQPHQALE GELLEHLSPG LALGAMAGPD GAVVSTPAHA PHMATMNPMH QAALSMAHAH241GLPSHMGCMS DVDADPRDLE AFAERFKQRR IKLGVTQADV GSALANLKIP GVGSLSQSTI301CRFESLTLSH NNMIALKPIL QAWLEEAEKS HREKLTKPEL FNGAEKKRKR TSIAAPEKRS361LEAYFAIQPR PSSEKIAAIA EKLDLKKNVV RVWFCNQRQK QKRMKYSAGITRTRPLEQKLISEEDLAANDILDYKDDDDKV
410 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
