Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BRN3B / POU4F2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116553-100
Catalog Number: LS-G116553
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POU4F2(POU4F2) (NM_004575), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brn-3b | BRN3.2 | POU class 4 homeobox 2 | POU4F2 | POU domain protein | Brain-3B | BRN3B
- Origin Species
- Human
- NCBI GenBank
- NM_004575
- SwissProt
- Q12837
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MMMMSLNSKQ AFSMPHGGSL HVEPKYSALH STSPGSSAPI APSASSPSSS SNAGGGGGGG61GGGGGGGGGR SSSSSSSGSS GGGGSEAMRR ACLPTPPSNI FGGLDESLLA RAEALAAVDI121VSQSKSHHHH PPHHSPFKPD ATYHTMNTIP CTSAASSSSV PISHPCALAG THHHHHHHHH181HHHQPHQALE GELLEHLSPG LALGAMAGPD GAVVSTPAHA PHMATMNPMH QAALSMAHAH241GLPSHMGCMS DVDADPRDLE AFAERFKQRR IKLGVTQADV GSALANLKIP GVGSLSQSTI301CRFESLTLSH NNMIALKPIL QAWLEEAEKS HREKLTKPEL FNGAEKKRKR TSIAAPEKRS361LEAYFAIQPR PSSEKIAAIA EKLDLKKNVV RVWFCNQRQK QKRMKYSAGITRTRPLEQKLISEEDLAANDILDYKDDDDKV
410 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.