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Human BF1 / FOXG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human BF1 / FOXG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FOXG1 antibodies →
$153.00each
Catalog Number: LS-G79214-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FOXG1 (NM_005249) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BF1 | BF2 | Brain factor 1 | BF-2 | FKHL1 | FKHL2 | Forkhead box G1A | Forkhead box protein G1 | Forkhead box protein G1A | Forkhead-related protein FKHL1 | Forkhead-related protein FKHL2 | FOXG1A | FOXG1B | FOXG1C | Forkhead box protein G1B | Forkhead-like 2 | Forkhead-like 4 | FOXG1 | HBF2 | HBF-1 | HFK1 | FKH2 | FKHL3 | FKHL4 | Forkhead box G1 | Forkhead box G1B | Forkhead box G1C | Forkhead-like 1 | Forkhead-related protein FKHL3 | HBF-3 | HBF-G2 | HFK3 | Transcription factor bf-2 | Oncogene QIN | QIN | BF-1 | Brain factor 2 | FHKL3 | Forkhead box protein G1C | Forkhead-like 3 | HBF-2 | HFK2 | KHL2
Origin Species
Human
NCBI GenBank
NM_005249
SwissProt
P55316

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MLDMGDRKEV KMIPKSSFSI NSLVPEAVQN DNHHASHGHH NSHHPQHHHH HHHHHHHPPP
61PAPQPPPPPQ QQQPPPPPPP APQPPQTRGA PAADDDKGPQ QLLLPPPPPP PPAAALDGAK
121AVGLGGKGEP GGGPGELAPV GPDEKEKGAG AGGEEKKGAG EGGKDGEGGK EGEKKNGKYE
181KPPFSYNALI MMAIRQSPEK RLTLNGIYEF IMKNFPYYRE NKQGWQNSIR HNLSLNMCFV
241KVPRHYDDPG KGNYWMLDPS SDDVFIGGTT GKLRRRSTTS RAKLAFKRGA RLTSTGLTFM
301DRAGSLYWPM SPFLSLHHPR ASSTLSYNGT TSAYPSHPMP YSSVLTQNSL GNNHSFSTAN
361GLSVDRLVNG EIPYATHHLT AAALAASVPC GLSVPCSGTY SLNPCSVNLL AGQTSYFFPH
421VPHPSMTSQS STSMSARATS SSTSPQAPST LPCESLRPSL PSFTTGLSGG LSDYFTHQNQ
481GSSSNPLIH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

489 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.