Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BF1 / FOXG1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116085-100
Catalog Number: LS-G116085
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FOXG1 (NM_005249), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BF1 | BF2 | Brain factor 1 | BF-2 | FKHL1 | FKHL2 | Forkhead box G1A | Forkhead box protein G1 | Forkhead box protein G1A | Forkhead-related protein FKHL1 | Forkhead-related protein FKHL2 | FOXG1A | FOXG1B | FOXG1C | Forkhead box protein G1B | Forkhead-like 2 | Forkhead-like 4 | FOXG1 | HBF2 | HBF-1 | HFK1 | FKH2 | FKHL3 | FKHL4 | Forkhead box G1 | Forkhead box G1B | Forkhead box G1C | Forkhead-like 1 | Forkhead-related protein FKHL3 | HBF-3 | HBF-G2 | HFK3 | Transcription factor bf-2 | Oncogene QIN | QIN | BF-1 | Brain factor 2 | FHKL3 | Forkhead box protein G1C | Forkhead-like 3 | HBF-2 | HFK2 | KHL2
- Origin Species
- Human
- NCBI GenBank
- NM_005249
- SwissProt
- P55316
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLDMGDRKEV KMIPKSSFSI NSLVPEAVQN DNHHASHGHH NSHHPQHHHH HHHHHHHPPP61PAPQPPPPPQ QQQPPPPPPP APQPPQTRGA PAADDDKGPQ QLLLPPPPPP PPAAALDGAK121AVGLGGKGEP GGGPGELAPV GPDEKEKGAG AGGEEKKGAG EGGKDGEGGK EGEKKNGKYE181KPPFSYNALI MMAIRQSPEK RLTLNGIYEF IMKNFPYYRE NKQGWQNSIR HNLSLNMCFV241KVPRHYDDPG KGNYWMLDPS SDDVFIGGTT GKLRRRSTTS RAKLAFKRGA RLTSTGLTFM301DRAGSLYWPM SPFLSLHHPR ASSTLSYNGT TSAYPSHPMP YSSVLTQNSL GNNHSFSTAN361GLSVDRLVNG EIPYATHHLT AAALAASVPC GLSVPCSGTY SLNPCSVNLL AGQTSYFFPH421VPHPSMTSQS STSMSARATS SSTSPQAPST LPCESLRPSL PSFTTGLSGG LSDYFTHQNQ481GSSSNPLIHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
489 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.