LSBio
Human BAF53 / ACTL6A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human BAF53 / ACTL6A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ACTL6A antibodies →
$153.00each
Catalog Number: LS-G87239-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human BAF53A(ACTL6A) (NM_177989) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACTL6 | Actin-like 6A | Actin-related protein 4 | Actin-related protein Baf53a | ArpNbeta | BAF53 | ARPN-BETA | BAF53A | BRG1-associated factor 53A | Actin-like protein 6A | ACTL6A | INO80 complex subunit K | ARP4 | BAF complex 53 kDa subunit | HArpN beta | INO80K
Origin Species
Human
NCBI GenBank
NM_004301
SwissProt
O96019

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
43.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVVERDDGST LMEIDGDKGK QGGPTYYIDT NALRVPRENM EAISPLKNGM VEDWDSFQAI
61LDHTYKMHVK SEASLHPVLM SEAPWNTRAK REKLTELMFE HYNIPAFFLC KTAVLTAFAN
121GRSTGLILDS GATHTTAIPV HDGYVLQQGI VKSPLAGDFI TMQCRELFQE MNIELVPPYM
181IASKEAVREG SPANWKRKEK LPQVTRSWHN YMCNCVIQDF QASVLQVSDS TYDEQVAAQM
241PTVHYEFPNG YNCDFGAERL KIPEGLFDPS NVKGLSGNTM LGVSHVVTTS VGMCDIDIRP
301GLYGSVIVAG GNTLIQSFTD RLNRELSQKT PPSMRLKLIA NNTTVERRFS SWIGGSILAS
361LGTFQQMWIS KQEYEEGGKQ CVERKCP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

387 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.