Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BAF53 / ACTL6A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104820-100
Catalog Number: LS-G104820
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BAF53A(ACTL6A) (NM_177989), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACTL6 | Actin-like 6A | Actin-related protein 4 | Actin-related protein Baf53a | ArpNbeta | BAF53 | ARPN-BETA | BAF53A | BRG1-associated factor 53A | Actin-like protein 6A | ACTL6A | INO80 complex subunit K | ARP4 | BAF complex 53 kDa subunit | HArpN beta | INO80K
- Origin Species
- Human
- NCBI GenBank
- NM_004301
- SwissProt
- O96019
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVVERDDGST LMEIDGDKGK QGGPTYYIDT NALRVPRENM EAISPLKNGM VEDWDSFQAI61LDHTYKMHVK SEASLHPVLM SEAPWNTRAK REKLTELMFE HYNIPAFFLC KTAVLTAFAN121GRSTGLILDS GATHTTAIPV HDGYVLQQGI VKSPLAGDFI TMQCRELFQE MNIELVPPYM181IASKEAVREG SPANWKRKEK LPQVTRSWHN YMCNCVIQDF QASVLQVSDS TYDEQVAAQM241PTVHYEFPNG YNCDFGAERL KIPEGLFDPS NVKGLSGNTM LGVSHVVTTS VGMCDIDIRP301GLYGSVIVAG GNTLIQSFTD RLNRELSQKT PPSMRLKLIA NNTTVERRFS SWIGGSILAS361LGTFQQMWIS KQEYEEGGKQ CVERKCPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
387 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.