AURKB / Aurora-B Antibody (clone AURKB/1521)
Catalog Number: LS-C761366
2 sizes / from $379.00
Antibody: Mouse Monoclonal
Applications: Flow Cytometry, Immunofluorescence, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human AURKB / Aurora-B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G80048-20
Catalog Number: LS-G80048
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Aurora B(AURKB) (NM_004217) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AIRK2 | AIK2 | Aurora kinase B | Aurora kinase B-Sv1 | Aurora-related kinase 2 | Aurora/IPL1-related kinase 2 | ARK-2 | ARK2 | Aurkb-sv1 | Aurkb-sv2 | Aurora 1 | Aurora-B | AIM-1 | IPL1 | STK-1 | STK5 | PPP1R48 | AurB | AURKB | Aurora B | Aurora kinase B-Sv2 | Aurora-1 | Aurora1 | Serine/threonine kinase 12 | STK1 | STK12
- Origin Species
- Human
- NCBI GenBank
- NM_004217
- SwissProt
- Q96GD4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT AAPGQKVMEN61SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH FIVALKVLFK SQIEKEGVEH121QLRREIEIQA HLHHPNILRL YNYFYDRRRI YLILEYAPRG ELYKELQKSC TFDEQRTATI181MEELADALMY CHGKKVIHRD IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL241PPEMIEGRMH NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPTGA301QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVATRTRPLEQKLISEEDLAANDILDYKDDDDKV
344 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
