Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AURKB / Aurora-B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116802-100
Catalog Number: LS-G116802
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Aurora B(AURKB) (NM_004217), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AIRK2 | AIK2 | Aurora kinase B | Aurora kinase B-Sv1 | Aurora-related kinase 2 | Aurora/IPL1-related kinase 2 | ARK-2 | ARK2 | Aurkb-sv1 | Aurkb-sv2 | Aurora 1 | Aurora-B | AIM-1 | IPL1 | STK-1 | STK5 | PPP1R48 | AurB | AURKB | Aurora B | Aurora kinase B-Sv2 | Aurora-1 | Aurora1 | Serine/threonine kinase 12 | STK1 | STK12
- Origin Species
- Human
- NCBI GenBank
- NM_004217
- SwissProt
- Q96GD4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT AAPGQKVMEN61SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH FIVALKVLFK SQIEKEGVEH121QLRREIEIQA HLHHPNILRL YNYFYDRRRI YLILEYAPRG ELYKELQKSC TFDEQRTATI181MEELADALMY CHGKKVIHRD IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL241PPEMIEGRMH NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPTGA301QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVATRTRPLEQKLISEEDLAANDILDYKDDDDKV
344 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.