LSBio
Human ARSK / Arylsulfatase K Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ARSK / Arylsulfatase K Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ARSK antibodies →
$220.00each
Catalog Number: LS-G93845-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ARSK (NM_198150) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Arylsulfatase family | member K | Arylsulfatase K | TSULF | ARSK | ASK | Telethon sulfatase
Origin Species
Human
SwissProt
Q6UWY0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLLWVSVVA ALALAVLAPG AGEQRRRAAK APNVVLVVSD SFDGRLTFHP GSQVVKLPFI
61NFMKTRGTSF LNAYTNSPIC CPSRAAMWSG LFTHLTESWN NFKGLDPNYT TWMDVMERHG
121YRTQKFGKLD YTSGHHSISN RVEAWTRDVA FLLRQEGRPM VNLIRNRTKV RVMERDWQNT
181DKAVNWLRKE AINYTEPFVI YLGLNLPHPY PSPSSGENFG SSTFHTSLYW LEKVSHDAIK
241IPKWSPLSEM HPVDYYSSYT KNCTGRFTKK EIKNIRAFYY AMCAETDAML GEIILALHQL
301DLLQKTIVIY SSDHGELAME HRQFYKMSMY EASAHVPLLM MGPGIKAGLQ VSNVVSLVDI
361YPTMLDIAGI PLPQNLSGYS LLPLSSETFK NEHKVKNLHP PWILSEFHGC NVNASTYMLR
421TNHWKYIAYS DGASILPQLF DLSSDPDELT NVAVKFPEIT YSLDQKLHSI INYPKVSASV
481HQYNKEQFIK WKQSIGQNYS NVIANLRWHQ DWQKEPRKYE NAIDQWLKTH MNPRAV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

536 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.