Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARSK / Arylsulfatase K Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103827-100
Catalog Number: LS-G103827
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARSK (NM_198150), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Arylsulfatase family | member K | Arylsulfatase K | TSULF | ARSK | ASK | Telethon sulfatase
- Origin Species
- Human
- SwissProt
- Q6UWY0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLLWVSVVA ALALAVLAPG AGEQRRRAAK APNVVLVVSD SFDGRLTFHP GSQVVKLPFI61NFMKTRGTSF LNAYTNSPIC CPSRAAMWSG LFTHLTESWN NFKGLDPNYT TWMDVMERHG121YRTQKFGKLD YTSGHHSISN RVEAWTRDVA FLLRQEGRPM VNLIRNRTKV RVMERDWQNT181DKAVNWLRKE AINYTEPFVI YLGLNLPHPY PSPSSGENFG SSTFHTSLYW LEKVSHDAIK241IPKWSPLSEM HPVDYYSSYT KNCTGRFTKK EIKNIRAFYY AMCAETDAML GEIILALHQL301DLLQKTIVIY SSDHGELAME HRQFYKMSMY EASAHVPLLM MGPGIKAGLQ VSNVVSLVDI361YPTMLDIAGI PLPQNLSGYS LLPLSSETFK NEHKVKNLHP PWILSEFHGC NVNASTYMLR421TNHWKYIAYS DGASILPQLF DLSSDPDELT NVAVKFPEIT YSLDQKLHSI INYPKVSASV481HQYNKEQFIK WKQSIGQNYS NVIANLRWHQ DWQKEPRKYE NAIDQWLKTH MNPRAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
536 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.