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Human ARSJ / Arylsulfatase J Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ARSJ / Arylsulfatase J Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G85464-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Arylsulfatase J(ARSJ) (NM_024590) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ASJ | ARSJ | Arylsulfatase family | member J | Arylsulfatase J
Origin Species
Human
NCBI GenBank
NM_024590
SwissProt
Q5FYB0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPRGCAGHP PPPSPQACVC PGKMLAMGAL AGFWILCLLT YGYLSWGQAL EEEEEGALLA
61QAGEKLEPST TSTSQPHLIF ILADDQGFRD VGYHGSEIKT PTLDKLAAEG VKLENYYVQP
121ICTPSRSQFI TGKYQIHTGL QHSIIRPTQP NCLPLDNATL PQKLKEVGYS THMVGKWHLG
181FYRKECMPTR RGFDTFFGSL LGSGDYYTHY KCDSPGMCGY DLYENDNAAW DYDNGIYSTQ
241MYTQRVQQIL ASHNPTKPIF LYIAYQAVHS PLQAPGRYFE HYRSIININR RRYAAMLSCL
301DEAINNVTLA LKTYGFYNNS IIIYSSDNGG QPTAGGSNWP LRSSKGTYWE GGIRAVGFVH
361SPLLKNKGTV CKELVHITDW YPTLISLAEG QIDEDIQLDG YDIWETISEG LRSPRVDILH
421NIDPIYTKAK NGSWAAGYGI WNTAIQSAIR VQHWKLLTGN PGYSDWVPPQ SFSNLGPNRW
481HNERITLSTG KSVWLFNITA DPYERVDLSN RYPGIVKKLL RRLSQFNKTA VPVRYPPKDP
541RSNPRLNGGV WGPWYKEETK KKKPSKNQAE KKQKKSKKKK KKQQKAVSGS TCHSGVTCG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

599 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.