Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARSJ / Arylsulfatase J Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101891-100
Catalog Number: LS-G101891
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Arylsulfatase J(ARSJ) (NM_024590), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASJ | ARSJ | Arylsulfatase family | member J | Arylsulfatase J
- Origin Species
- Human
- NCBI GenBank
- NM_024590
- SwissProt
- Q5FYB0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPRGCAGHP PPPSPQACVC PGKMLAMGAL AGFWILCLLT YGYLSWGQAL EEEEEGALLA61QAGEKLEPST TSTSQPHLIF ILADDQGFRD VGYHGSEIKT PTLDKLAAEG VKLENYYVQP121ICTPSRSQFI TGKYQIHTGL QHSIIRPTQP NCLPLDNATL PQKLKEVGYS THMVGKWHLG181FYRKECMPTR RGFDTFFGSL LGSGDYYTHY KCDSPGMCGY DLYENDNAAW DYDNGIYSTQ241MYTQRVQQIL ASHNPTKPIF LYIAYQAVHS PLQAPGRYFE HYRSIININR RRYAAMLSCL301DEAINNVTLA LKTYGFYNNS IIIYSSDNGG QPTAGGSNWP LRSSKGTYWE GGIRAVGFVH361SPLLKNKGTV CKELVHITDW YPTLISLAEG QIDEDIQLDG YDIWETISEG LRSPRVDILH421NIDPIYTKAK NGSWAAGYGI WNTAIQSAIR VQHWKLLTGN PGYSDWVPPQ SFSNLGPNRW481HNERITLSTG KSVWLFNITA DPYERVDLSN RYPGIVKKLL RRLSQFNKTA VPVRYPPKDP541RSNPRLNGGV WGPWYKEETK KKKPSKNQAE KKQKKSKKKK KKQQKAVSGS TCHSGVTCGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
599 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.