ARID4B Antibody
Catalog Number: LS-C807209
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARID4B Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87270-20
Catalog Number: LS-G87270
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human BRCAA1(ARID4B) (NM_031371) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BRCAA1 | ARID4B | SIN3A-associated protein 180 | BCAA | Rb-binding protein homolog | RBBP1L1 | RBP1L1 | SAP180
- Origin Species
- Human
- NCBI GenBank
- NM_016374
- SwissProt
- Q4LE39
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 137.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKALDEPPYL TVGTDVSAKY RGAFCEAKIK TAKRLVKVKV TFRHDSSTVE VQDDHIKGPL61KVGAIVEVKN LDGAYQEAVI NKLTDASWYT VVFDDGDEKT LRRSSLCLKG ERHFAESETL121DQLPLTNPEH FGTPVIGKKT NRGRRSNHIP EEESSSSSSD EDEDDRKQID ELLGKVVCVD181YISLDKKKAL WFPALVVCPD CSDEIAVKKD NILVRSFKDG KFTSVPRKDV HEITSDTAPK241PDAVLKQAFE QALEFHKSRT IPANWKTELK EDSSSSEAEE EEEEEDDEKE KEDNSSEEEE301EIEPFPEERE NFLQQLYKFM EDRGTPINKR PVLGYRNLNL FKLFRLVHKL GGFDNIESGA361VWKQVYQDLG IPVLNSAAGY NVKCAYKKYL YGFEEYCRSA NIEFQMALPE KVVNKQCKEC421ENVKEIKVKE ENETEIKEIK MEEERNIIPR EEKPIEDEIE RKENIKPSLG SKKNLLESIP481THSDQEKEVN IKKPEDNENL DDKDDDTTRV DESLNIKVEA EEEKAKSGYD EWIKADKIVR541PADKNVPKIK HRKKIKNKLD KEKDKDEKYS PKNCKLRRLS KPPFQTNPSP EMVSKLDLTD601AKNSDTAHIK SIEITSILNG LQASESSAED SEQEDERGAQ DMDNNGKEES KIDHLTNNRN661DLISKEEQNS SSLLEENKVH ADLVISKPVS KSPERLRKDI EVLSEDTDYE EDEVTKKRKD721VKKDTTDKSS KPQIKRGKRR YCNTEECLKT GSPGKKEEKA KNKESLCMEN SSNSSSDEDE781EETKAKMTPT KKYNGLEEKR KSLRTTGFYS GFSEVAEKRI KLLNNSDERL QNSRAKDRKD841VWSSIQGQWP KKTLKELFSD SDTEAAASPP HPAPEEGVAE ESLQTVAEEE SCSPSVELEK901PPPVNVDSKP IEEKTVEVND RKAEFPSSGS NSVLNTPPTT PESPSSVTVT EGSRQQSSVT961VSEPLAPNQE EVRSIKSETD STIEVDSVAG ELQDLQSEGN SSPAGFDASV SSSSSNQPEP1021EHPEKACTGQ KRVKDAQGGG SSSKKQKRSH KATVVNNKKK GKGTNSSDSE ELSAGESITK1081SQPVKSVSTG MKSHSTKSPA RTQSPGKCGK NGDKDPDLKE PSNRLPKVYK WSFQMSDLEN1141MTSAERITIL QEKLQEIRKH YLSLKSEVAS IDRRRKRLKK KERESAATSS SSSSPSSSSI1201TAAVMLTLAE PSMSSASQNG MSVECRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1226 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
