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ARID4B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARID4B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARID4B antibodies →
$725.00each
Catalog Number: LS-G109243-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRCAA1(ARID4B) (NM_031371), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BRCAA1 | ARID4B | SIN3A-associated protein 180 | BCAA | Rb-binding protein homolog | RBBP1L1 | RBP1L1 | SAP180
Origin Species
Human
NCBI GenBank
NM_016374
SwissProt
Q4LE39

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
137.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKALDEPPYL TVGTDVSAKY RGAFCEAKIK TAKRLVKVKV TFRHDSSTVE VQDDHIKGPL
61KVGAIVEVKN LDGAYQEAVI NKLTDASWYT VVFDDGDEKT LRRSSLCLKG ERHFAESETL
121DQLPLTNPEH FGTPVIGKKT NRGRRSNHIP EEESSSSSSD EDEDDRKQID ELLGKVVCVD
181YISLDKKKAL WFPALVVCPD CSDEIAVKKD NILVRSFKDG KFTSVPRKDV HEITSDTAPK
241PDAVLKQAFE QALEFHKSRT IPANWKTELK EDSSSSEAEE EEEEEDDEKE KEDNSSEEEE
301EIEPFPEERE NFLQQLYKFM EDRGTPINKR PVLGYRNLNL FKLFRLVHKL GGFDNIESGA
361VWKQVYQDLG IPVLNSAAGY NVKCAYKKYL YGFEEYCRSA NIEFQMALPE KVVNKQCKEC
421ENVKEIKVKE ENETEIKEIK MEEERNIIPR EEKPIEDEIE RKENIKPSLG SKKNLLESIP
481THSDQEKEVN IKKPEDNENL DDKDDDTTRV DESLNIKVEA EEEKAKSGYD EWIKADKIVR
541PADKNVPKIK HRKKIKNKLD KEKDKDEKYS PKNCKLRRLS KPPFQTNPSP EMVSKLDLTD
601AKNSDTAHIK SIEITSILNG LQASESSAED SEQEDERGAQ DMDNNGKEES KIDHLTNNRN
661DLISKEEQNS SSLLEENKVH ADLVISKPVS KSPERLRKDI EVLSEDTDYE EDEVTKKRKD
721VKKDTTDKSS KPQIKRGKRR YCNTEECLKT GSPGKKEEKA KNKESLCMEN SSNSSSDEDE
781EETKAKMTPT KKYNGLEEKR KSLRTTGFYS GFSEVAEKRI KLLNNSDERL QNSRAKDRKD
841VWSSIQGQWP KKTLKELFSD SDTEAAASPP HPAPEEGVAE ESLQTVAEEE SCSPSVELEK
901PPPVNVDSKP IEEKTVEVND RKAEFPSSGS NSVLNTPPTT PESPSSVTVT EGSRQQSSVT
961VSEPLAPNQE EVRSIKSETD STIEVDSVAG ELQDLQSEGN SSPAGFDASV SSSSSNQPEP
1021EHPEKACTGQ KRVKDAQGGG SSSKKQKRSH KATVVNNKKK GKGTNSSDSE ELSAGESITK
1081SQPVKSVSTG MKSHSTKSPA RTQSPGKCGK NGDKDPDLKE PSNRLPKVYK WSFQMSDLEN
1141MTSAERITIL QEKLQEIRKH YLSLKSEVAS IDRRRKRLKK KERESAATSS SSSSPSSSSI
1201TAAVMLTLAE PSMSSASQNG MSVECR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1226 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.