LSBio
Human ARHGEF9 / Collybistin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ARHGEF9 / Collybistin Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ARHGEF9 antibodies →
$153.00each
Catalog Number: LS-G79869-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF9 (NM_001173479) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHDH9 | EIEE8 | HPEM-2 | KIAA0424 | PEM-2 homolog | PEM2 | ARHGEF9 | COLLYBISTIN | HPEM-2 collybistin | PEM-2
Origin Species
Human
NCBI GenBank
NM_015185
SwissProt
O43307

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MQWIRGGSGM LWVNQEDEVE EGPSDVQNGH LDPNSDCLCL GRPLQNRDQM RANVINEIMS
61TERHYIKHLK DICEGYLKQC RKRRDMFSDE QLKVIFGNIE DIYRFQMGFV RDLEKQYNND
121DPHLSEIGPC FLEHQDGFWI YSEYCNNHLD ACMELSKLMK DSRYQHFFEA CRLLQQMIDI
181AIDGFLLTPV QKICKYPLQL AELLKYTAQD HSDYRYVAAA LAVMRNVTQQ INERKRRLEN
241IDKIAQWQAS VLDWEGEDIL DRSSELIYTG EMAWIYQPYG RNQQRVFFLF DHQMVLCKKD
301LIRRDILYYK GRIDMDKYEV VDIEDGRDDD FNVSMKNAFK LHNKETEEIH LFFAKKLEEK
361IRWLRAFREE RKMVQEDEKI GFEISENQKR QAAMTVRKVP KQKGVNSARS VPPSYPPPQD
421PLNHGQYLVP DGIAQSQVFE FTEPKRSQSP FWQNFSRLTP FKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

463 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.