Human ARHGEF35 Protein (Recombinant Myc-DDK (Flag))
Catalog Number: LS-G73541-20
20 µg / $1130.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARHGEF35 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95542-20
Catalog Number: LS-G95542
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF35 (NM_001003702) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF5L | ARHGEF35
- Origin Species
- Human
- SwissProt
- A5YM69
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAEEAQHGA SPPISAIEEF SIIPEAPMRS SQVSALGLEA QEDEDPSYKW REEHRLSATQ61QSELRDVCDY AIETMPSFPK EGSADVEPNQ ESLVAEACDT PEHWEAVPQS LAGRQARTLA121PPELWACPIQ SEHLDMAPFS SDLGSEEEEV EFWPGLTSLT LGSGQAEEEE ETSSDNSGQT181RYYSPCEEHP AETNQNEGAE SGTIRQGEEL PSEELQESQG LLHPQEVQVL EEQGQQEAGF241RGEGTLREDV CADGLLGEEQ MIEQVNDEKG EQKQKQEQVQ DVMLGRQGER MGLTGEPEGL301NDGEWEQEDM ERKAQGQGGP EQGEERKREL QVPEENRADS QDEKSQTFLG KSEEVTGKQE361DHGIKEKGVP VSGQEAKEPE SWDGGRLGAV GRARSREEEN EHHGPSMPAL IAPEDSPHCD421LFPGASYLVT QIPGTQTESR AEELSPAALS PLLEPIRCSH QPISLLGSFL TEESPDKEKL481LSVLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
484 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
