Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti-ECT2 pT790 Antibody - Western Blot. Western blot of affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1000. Personal Communication, Toru Miki, CCR-NCI, Bethesda, MD.
ECT2 Antibody (phospho-Thr790)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C19111-100
Catalog Number: LS-C19111
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- ECT2 antibody LS-C19111 is an unconjugated rabbit polyclonal antibody to ECT2 (pThr790) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Chicken, Dog, Mouse, Non-Human Primate, Rat, Zebrafish
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ARHGEF31 | ECT2 | Protein ECT2
- Recommended Dilution: ELISA
- 1:10000 - 1:40000
- Recommended Dilution: WB
- 1:1000 - 1:4000
- UniProt Number
- Q9H8V3
- NCBI GenBank
- NM_018098 | NP_060568.3
- SwissProt
- Q9H8V3
Target
- Target
- Human ECT2
- Antigen Species
- Human
- Epitope
- Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2. Additional experiments are underway to clarify antibody specificity. A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee, chicken, and dog based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Gene Name
- ECT2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
