ARHGEF25 / GEFT Antibody
Catalog Number: LS-C754910-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARHGEF25 / GEFT Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G86910-20
Catalog Number: LS-G86910
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GEFT(ARHGEF25) (NM_001111270) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF25 | GEFT | RhoA/RAC/CDC42 exchange factor | p63RhoGEF | RAC/CDC42 exchange factor
- Origin Species
- Human
- NCBI GenBank
- AA463333
- SwissProt
- Q86VW2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKPPDRPAPG RTDRILGVMG GMLRACALPG QEGPPRRSPL GLVGTEPESE RTEGDHRRDR61EHEVLAGALQ PESYSIAGSE GSISASAASG LAAPSGPSSG LSSGPCSPGP PGPVSGLRRW121LDHSKHCLSV ETEADSGQAG PYENWMLEPA LATGEELPEL TLLTTLLEGP GDKTQPPEEE181TLSQAPESEE EQKKKALERS MYVLSELVET EKMYVDDLGQ IVEGYMATMA AQGVPESLRG241RDRIVFGNIQ QIYEWHRDYF LQELQRCLKD PDWLAQLFIK HERRLHMYVV YCQNKPKSEH301VVSEFGDSYF EELRQQLGHR LQLNDLLIKP VQRIMKYQLL LKDFLKYYNR AGMDTADLEQ361AVEVMCFVPK RCNDMMTLGR LRGFEGKLTA QGKLLGQDTF WVTEPEAGGL LSSRGRERRV421FLFEQIIIFS EALGGGVRGG TQPGYVYKNS IKVSCLGLEG NLQGDPCRFA LTSRGPEGGI481QRYVLQAADP AISQAWIKHV AQILESQRDF LNALQSPIEY QRRESQTNSL GRPRGPGVGS541PGRIRLGDQA QGSTHTPING SLPSLLLSPK GEVARALLPL DKQALGDIPQ APHDSPPVSP601TPKTPPCQAR LAKLDEDELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
619 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
