Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARHGEF25 / GEFT Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124232-100
Catalog Number: LS-G124232
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GEFT(ARHGEF25) (NM_001111270), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF25 | GEFT | RhoA/RAC/CDC42 exchange factor | p63RhoGEF | RAC/CDC42 exchange factor
- Origin Species
- Human
- NCBI GenBank
- AA463333
- SwissProt
- Q86VW2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKPPDRPAPG RTDRILGVMG GMLRACALPG QEGPPRRSPL GLVGTEPESE RTEGDHRRDR61EHEVLAGALQ PESYSIAGSE GSISASAASG LAAPSGPSSG LSSGPCSPGP PGPVSGLRRW121LDHSKHCLSV ETEADSGQAG PYENWMLEPA LATGEELPEL TLLTTLLEGP GDKTQPPEEE181TLSQAPESEE EQKKKALERS MYVLSELVET EKMYVDDLGQ IVEGYMATMA AQGVPESLRG241RDRIVFGNIQ QIYEWHRDYF LQELQRCLKD PDWLAQLFIK HERRLHMYVV YCQNKPKSEH301VVSEFGDSYF EELRQQLGHR LQLNDLLIKP VQRIMKYQLL LKDFLKYYNR AGMDTADLEQ361AVEVMCFVPK RCNDMMTLGR LRGFEGKLTA QGKLLGQDTF WVTEPEAGGL LSSRGRERRV421FLFEQIIIFS EALGGGVRGG TQPGYVYKNS IKVSCLGLEG NLQGDPCRFA LTSRGPEGGI481QRYVLQAADP AISQAWIKHV AQILESQRDF LNALQSPIEY QRRESQTNSL GRPRGPGVGS541PGRIRLGDQA QGSTHTPING SLPSLLLSPK GEVARALLPL DKQALGDIPQ APHDSPPVSP601TPKTPPCQAR LAKLDEDELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
619 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.