ARHGEF2 / GEF-H1 Antibody
Catalog Number: LS-C665807-100
100 µg / $359.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARHGEF2 / GEF-H1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G81768-20
Catalog Number: LS-G81768
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GEF H1(ARHGEF2) (NM_004723) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF2 | GEF H1 | GEFH1 | GEF-H1 | LFP40 | Microtubule-regulated Rho-GEF | KIAA0651 | GEF | p40
- Origin Species
- Human
- NCBI GenBank
- NM_004723
- SwissProt
- Q92974
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 108.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKEAKDARYT NGHLFTTISV SGMTMCYACN KSITAKEALI CPTCNVTIHN RCKDTLANCT61KVKQKQQKAA LLKNNTALQS VSLRSKTTIR ERPSSAIYPS DSFRQSLLGS RRGRSSLSLA121KSVSTTNIAG HFNDESPLGL RRILSQSTDS LNMRNRTLSV ESLIDEAEVI YSELMSDFEM181DEKDFAADSW SLAVDSSFLQ QHKKEVMKQQ DVIYELIQTE LHHVRTLKIM TRLFRTGMLE241ELHLEPGVVQ GLFPCVDELS DIHTRFLSQL LERRRQALCP GSTRNFVIHR LGDLLISQFS301GPSAEQMCKT YSEFCSRHSK ALKLYKELYA RDKRFQQFIR KVTRPAVLKR HGVQECILLV361TQRITKYPLL ISRILQHSHG IEEERQDLTT ALGLVKELLS NVDEGIYQLE KGARLQEIYN421RMDPRAQTPV PGKGPFGREE LLRRKLIHDG CLLWKTATGR FKDVLVLLMT DVLVFLQEKD481QKYIFPTLDK PSVVSLQNLI VRDIANQEKG MFLISAAPPE MYEVHTASRD DRSTWIRVIQ541QSVRTCPSRE DFPLIETEDE AYLRRIKMEL QQKDRALVEL LREKVGLFAE MTHFQAEEDG601GSGMALPTLP RGLFRSESLE SPRGERLLQD AIREVEGLKD LLVGPGVELL LTPREPALPL661EPDSGGNTSP GVTANGEART FNGSIELCRA DSDSSQRDRN GNQLRSPQEE ALQRLVNLYG721LLHGLQAAVA QQDTLMEARF PEGPERREKL CRANSRDGEA GRAGAAPVAP EKQATELALL781QRQHALLQEE LRRCRRLGEE RATEAGSLEA RLRESEQARA LLEREAEEAR RQLAALGQTE841PLPAEAPWAR RPVDPRRRSL PAGDALYLSF NPPQPSRGTD RLDLPVTTRS VHRNFEDRER901QELGSPEERL QDSSDPDTGS EEEGSSRLSP PHSPRDFTRM QDIPEETESR DGEAVASESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
959 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
