Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARHGEF2 / GEF-H1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116454-100
Catalog Number: LS-G116454
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GEF H1(ARHGEF2) (NM_004723), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF2 | GEF H1 | GEFH1 | GEF-H1 | LFP40 | Microtubule-regulated Rho-GEF | KIAA0651 | GEF | p40
- Origin Species
- Human
- NCBI GenBank
- NM_004723
- SwissProt
- Q92974
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 108.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKEAKDARYT NGHLFTTISV SGMTMCYACN KSITAKEALI CPTCNVTIHN RCKDTLANCT61KVKQKQQKAA LLKNNTALQS VSLRSKTTIR ERPSSAIYPS DSFRQSLLGS RRGRSSLSLA121KSVSTTNIAG HFNDESPLGL RRILSQSTDS LNMRNRTLSV ESLIDEAEVI YSELMSDFEM181DEKDFAADSW SLAVDSSFLQ QHKKEVMKQQ DVIYELIQTE LHHVRTLKIM TRLFRTGMLE241ELHLEPGVVQ GLFPCVDELS DIHTRFLSQL LERRRQALCP GSTRNFVIHR LGDLLISQFS301GPSAEQMCKT YSEFCSRHSK ALKLYKELYA RDKRFQQFIR KVTRPAVLKR HGVQECILLV361TQRITKYPLL ISRILQHSHG IEEERQDLTT ALGLVKELLS NVDEGIYQLE KGARLQEIYN421RMDPRAQTPV PGKGPFGREE LLRRKLIHDG CLLWKTATGR FKDVLVLLMT DVLVFLQEKD481QKYIFPTLDK PSVVSLQNLI VRDIANQEKG MFLISAAPPE MYEVHTASRD DRSTWIRVIQ541QSVRTCPSRE DFPLIETEDE AYLRRIKMEL QQKDRALVEL LREKVGLFAE MTHFQAEEDG601GSGMALPTLP RGLFRSESLE SPRGERLLQD AIREVEGLKD LLVGPGVELL LTPREPALPL661EPDSGGNTSP GVTANGEART FNGSIELCRA DSDSSQRDRN GNQLRSPQEE ALQRLVNLYG721LLHGLQAAVA QQDTLMEARF PEGPERREKL CRANSRDGEA GRAGAAPVAP EKQATELALL781QRQHALLQEE LRRCRRLGEE RATEAGSLEA RLRESEQARA LLEREAEEAR RQLAALGQTE841PLPAEAPWAR RPVDPRRRSL PAGDALYLSF NPPQPSRGTD RLDLPVTTRS VHRNFEDRER901QELGSPEERL QDSSDPDTGS EEEGSSRLSP PHSPRDFTRM QDIPEETESR DGEAVASESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
959 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.