ARHGEF18 Antibody
Catalog Number: LS-C659775-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARHGEF18 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G79917-20
Catalog Number: LS-G79917
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human p114RhoGEF(ARHGEF18) (NM_001130955) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- p114-RhoGEF | KIAA0521 | SA-RhoGEF | Septin-associated RhoGEF | p114RhoGEF | ARHGEF18 | p114-RHO-GEF
- Origin Species
- Human
- NCBI GenBank
- NM_015318
- SwissProt
- Q6ZSZ5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 130.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVTVGTNILP SRPAASANTA REDAALFSRR IPPRHKNGAA QPGAAPGPGA PGANMGNAHS61KSGDRHSALP GRPELSFYGS FPRKWSENVF LDNELLTSKI LSVLRPQSER GFRAGDLRYP121THFLSTNSVL ASVTASLKEH PRGTLLSDGS PALSRNVGMT VSQKGGPQPT PSPAGPGTQL181GPITGEMDEA DSAFLKFKQT ADDSLSLTSP NTESIFVEDP YTASLRSEIE SDGHEFEAES241WSLAVDAAYA KKQKREVVKR QDVLYELMQT EVHHVRTLKI MLKVYSRALQ EELQFSSKAI301GRLFPCADDL LETHSHFLAR LKERRQESLE EGSDRNYVIQ KIGDLLVQQF SGENGERMKE361KYGVFCSGHN EAVSHYKLLL QQNKKFQNLI KKIGNFSIVR RLGVQECILL VTQRITKYPV421LVERIIQNTE AGTEDYEDLT QALNLIKDII SQVDAKVSEC EKGQRLREIA GKMDLKSSSK481LKNGLTFRKE DMLQRQLHLE GMLCWKTTSG RLKDILAILL TDVLLLLQEK DQKYVFASVD541SKPPVISLQK LIVREVANEE KAMFLISASL QGPEMYEIYT SSKEDRNAWM AHIQRAVESC601PDEEEGPFSL PEEERKVVEA RATRLRDFQE RLSMKDQLIA QSLLEKQQIY LEMAEMGGLE661DLPQPRGLFR GGDPSETLQG ELILKSAMSE IEGIQSLICR QLGSANGQAE DGGSSTGPPR721RAETFAGYDC TNSPTKNGSF KKKVSSTDPR PRDWRGPPNS PDLKLSDSDI PGSSEESPQV781VEAPGTESDP RLPTVLESEL VQRIQTLSQL LLNLQAVIAH QDSYVETQRA AIQEREKQFR841LQSTRGNLLL EQERQRNFEK QREERAALEK LQSQLRHEQQ RWERERQWQH QELERAGARL901QEREGEARQL RERLEQERAE LERQRQAYQH DLERLREAQR AVERERERLE LLRRLKKQNT961APGALPPDTL AEAQPPSHPP SFNGEGLEGP RVSMLPSGVG PEYAERPEVA RRDSAPTENR1021LAKSDVPIQL LSATNQFQRQ AAVQQQIPTK LAASTKGGKD KGGKSRGSQR WESSASFDLK1081QQLLLNKLMG KDESTSRNRR SLSPILPGRH SPAPPPDPGF PAPSPPPADS PSEGFSLKAG1141GTALLPGPPA PSPLPATPLS AKEDASKEDV IFFSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1173 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
