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ARHGEF18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGEF18 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGEF18 antibodies →
$725.00each
Catalog Number: LS-G125165-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human p114RhoGEF(ARHGEF18) (NM_001130955), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
p114-RhoGEF | KIAA0521 | SA-RhoGEF | Septin-associated RhoGEF | p114RhoGEF | ARHGEF18 | p114-RHO-GEF
Origin Species
Human
NCBI GenBank
NM_015318
SwissProt
Q6ZSZ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
130.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVTVGTNILP SRPAASANTA REDAALFSRR IPPRHKNGAA QPGAAPGPGA PGANMGNAHS
61KSGDRHSALP GRPELSFYGS FPRKWSENVF LDNELLTSKI LSVLRPQSER GFRAGDLRYP
121THFLSTNSVL ASVTASLKEH PRGTLLSDGS PALSRNVGMT VSQKGGPQPT PSPAGPGTQL
181GPITGEMDEA DSAFLKFKQT ADDSLSLTSP NTESIFVEDP YTASLRSEIE SDGHEFEAES
241WSLAVDAAYA KKQKREVVKR QDVLYELMQT EVHHVRTLKI MLKVYSRALQ EELQFSSKAI
301GRLFPCADDL LETHSHFLAR LKERRQESLE EGSDRNYVIQ KIGDLLVQQF SGENGERMKE
361KYGVFCSGHN EAVSHYKLLL QQNKKFQNLI KKIGNFSIVR RLGVQECILL VTQRITKYPV
421LVERIIQNTE AGTEDYEDLT QALNLIKDII SQVDAKVSEC EKGQRLREIA GKMDLKSSSK
481LKNGLTFRKE DMLQRQLHLE GMLCWKTTSG RLKDILAILL TDVLLLLQEK DQKYVFASVD
541SKPPVISLQK LIVREVANEE KAMFLISASL QGPEMYEIYT SSKEDRNAWM AHIQRAVESC
601PDEEEGPFSL PEEERKVVEA RATRLRDFQE RLSMKDQLIA QSLLEKQQIY LEMAEMGGLE
661DLPQPRGLFR GGDPSETLQG ELILKSAMSE IEGIQSLICR QLGSANGQAE DGGSSTGPPR
721RAETFAGYDC TNSPTKNGSF KKKVSSTDPR PRDWRGPPNS PDLKLSDSDI PGSSEESPQV
781VEAPGTESDP RLPTVLESEL VQRIQTLSQL LLNLQAVIAH QDSYVETQRA AIQEREKQFR
841LQSTRGNLLL EQERQRNFEK QREERAALEK LQSQLRHEQQ RWERERQWQH QELERAGARL
901QEREGEARQL RERLEQERAE LERQRQAYQH DLERLREAQR AVERERERLE LLRRLKKQNT
961APGALPPDTL AEAQPPSHPP SFNGEGLEGP RVSMLPSGVG PEYAERPEVA RRDSAPTENR
1021LAKSDVPIQL LSATNQFQRQ AAVQQQIPTK LAASTKGGKD KGGKSRGSQR WESSASFDLK
1081QQLLLNKLMG KDESTSRNRR SLSPILPGRH SPAPPPDPGF PAPSPPPADS PSEGFSLKAG
1141GTALLPGPPA PSPLPATPLS AKEDASKEDV IFF
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1173 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.