ARHGAP27 Antibody (aa350-548)
Catalog Number: LS-C179087-100
100 µL (1 mg/mL) / $339.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARHGAP27 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G94741-20
Catalog Number: LS-G94741
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SH3D20(ARHGAP27) (NM_199282) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PP905 | SH3 domain containing 20 | ARHGAP27 | CAMGAP1 | SH3D20 | SH3P20
- Origin Species
- Human
- SwissProt
- Q6ZUM4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQPGLSPGSP GDPRPPTPET DYPESLTSYP EEDYSPVGSF GEPGPTSPLT TPPGWSCHVS61QDKQMLYTNH FTQEQWVRLE DPHGKPYFYN PEDSSVRWEL PQVPVPAPRS IHKSSQDGDT121PAQASPPEEK VPAELDEVGS WEEVSPATAA VRTKTLDKAG VLHRTKTADK GKRLRKKHWS181ASWTVLEGGV LTFFKDSKTS AAGGLRQPSK FSTPEYTVEL RGATLSWAPK DKSSRKNVLE241LRSRDGSEYL IQHDSEAIIS TWHKAIAQGI QELSAELPPE ESESSRVDFG SSERLGSWQE301KEEDARPNAA APALGPVGLE SDLSKVRHKL RKFLQRRPTL QSLREKGYIK DQVFGCALAA361LCERERSRVP RFVQQCIRAV EARGLDIDGL YRISGNLATI QKLRYKVDHD ERLDLDDGRW421EDVHVITGAL KLFFRELPEP LFPFSHFRQF IAAIKLQDQA RRSRCVRDLV RSLPAPNHDT481LRMLFQHLCR VIEHGEQNRM SVQSVAIVFG PTLLRPEVEE TSMPMTMVFQ NQVVELILQQ541CADIFPPHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
548 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
