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ARHGAP27 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP27 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP27 antibodies →
$725.00each
Catalog Number: LS-G103374-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SH3D20(ARHGAP27) (NM_199282), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PP905 | SH3 domain containing 20 | ARHGAP27 | CAMGAP1 | SH3D20 | SH3P20
Origin Species
Human
SwissProt
Q6ZUM4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MQPGLSPGSP GDPRPPTPET DYPESLTSYP EEDYSPVGSF GEPGPTSPLT TPPGWSCHVS
61QDKQMLYTNH FTQEQWVRLE DPHGKPYFYN PEDSSVRWEL PQVPVPAPRS IHKSSQDGDT
121PAQASPPEEK VPAELDEVGS WEEVSPATAA VRTKTLDKAG VLHRTKTADK GKRLRKKHWS
181ASWTVLEGGV LTFFKDSKTS AAGGLRQPSK FSTPEYTVEL RGATLSWAPK DKSSRKNVLE
241LRSRDGSEYL IQHDSEAIIS TWHKAIAQGI QELSAELPPE ESESSRVDFG SSERLGSWQE
301KEEDARPNAA APALGPVGLE SDLSKVRHKL RKFLQRRPTL QSLREKGYIK DQVFGCALAA
361LCERERSRVP RFVQQCIRAV EARGLDIDGL YRISGNLATI QKLRYKVDHD ERLDLDDGRW
421EDVHVITGAL KLFFRELPEP LFPFSHFRQF IAAIKLQDQA RRSRCVRDLV RSLPAPNHDT
481LRMLFQHLCR VIEHGEQNRM SVQSVAIVFG PTLLRPEVEE TSMPMTMVFQ NQVVELILQQ
541CADIFPPH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

548 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.