ARHGAP25 Antibody
Catalog Number: LS-C331364
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARHGAP25 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G76651-20
Catalog Number: LS-G76651
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP25 (NM_001166277) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGAP25 | KAIA0053 | KIAA0053
- Origin Species
- Human
- NCBI GenBank
- AC112700
- SwissProt
- P42331
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLKLPRNWD FNLKVEAAKI ARSRSVMTGE QMAAFHPSST PNPLERPIKM GWLKKQRSIV61KNWQQRYFVL RAQQLYYYKD EEDTKPQGCM YLPGCTIKEI ATNPEEAGKF VFEIIPVFGQ121RLDETVAYEQ KFGPHLVPIL VEKCAEFILE HGRNEEGIFR LPGQDNLVKQ LRDAFDAGER181PSFDRDTDVH TVASLLKLYL RDLPEPVVPW SQYEGFLLCG QLTNADEAKA QQELMKQLSI241LPRDNYSLLS YICRFLHEIQ LNCAVNKMSV DNLATVIGVN LIRSKVEDPA VIMRGTPQIQ301RVMTMMIRDH EVLFPKSKDI PLSPPAQKND PKKAPVARSS VGWDATEDLR ISRTDSFSSM361TSDSDTTSPT GQQPSDAFPE DSSKVPREKP GDWKMQSRKR TQTLPNRKCF LTSAFQGANS421SKMEIFKNEF WSPSSEAKAG EGHRRTMSQD LRQLSDSQRT STYDNVPSLP GSPGEEASAL481SSQACDSKGD TLASPNSETG PGKKNSGEEE IDSLQRMVQE LRKEIETQKQ MYEEQIKNLE541KENYDVWAKV VRLNEELEKE KKKSAALEIS LRNMERSRED VEKRNKALEE EVKEFVKSMK601EPKTEATRTRPLEQKLISEEDLAANDILDYKDDDDKV
606 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
