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ARHGAP25 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP25 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP25 antibodies →
$725.00each
Catalog Number: LS-G101269-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP25 (NM_014882), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP25 | KAIA0053 | KIAA0053
Origin Species
Human
NCBI GenBank
AC112700
SwissProt
P42331

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLGQSACLF LSIARSRSVM TGEQMAAFHP SSTPNPLERP IKMGWLKKQR SIVKNWQQRY
61FVLRAQQLYY YKDEEDTKPQ GCMYLPGCTI KEIATNPEEA GKFVFEIIPA SWDQNRMGQD
121SYVLMASSQA EMEEWVKFLR RVAGTPCGAV FGQRLDETVA YEQKFGPHLV PILVEKCAEF
181ILEHGRNEEG IFRLPGQDNL VKQLRDAFDA GERPSFDRDT DVHTVASLLK LYLRDLPEPV
241VPWSQYEGFL LCGQLTNADE AKAQQELMKQ LSILPRDNYS LLSYICRFLH EIQLNCAVNK
301MSVDNLATVI GVNLIRSKVE DPAVIMRGTP QIQRVMTMMI RDHEVLFPKS KDIPLSPPAQ
361KNDPKKAPVA RSSVGWDATE DLRISRTDSF SSMTSDSDTT SPTGQQPSDA FPEDSSKVPR
421EKPGDWKMQS RKRTQTLPNR KCFLTSAFQG ANSSKMEIFK NEFWSPSSEA KAGEGHRRTM
481SQDLRQLSDS QRTSTYDNVP SLPGSPGEEA SALSSQACDS KGDTLASPNS ETGPGKKNSG
541EEEIDSLQRT VQELRKEIET QKQMYEEQIK NLEKENYDVW AKVVRLNEEL EKEKKKSAAL
601EISLRNMERS REDVEKRNKA LEEEVKEFVK SMKEPKTEA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

639 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.