LSBio
Human AP1G1 / Adaptin Gamma 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human AP1G1 / Adaptin Gamma 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G82011-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human gamma Adaptin(AP1G1) (NM_001030007) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADTG | AP1G1 | AP-1 complex subunit gamma-1 | CLAPG1 | Gamma adaptin | Gamma1-adaptin
Origin Species
Human
NCBI GenBank
NM_001128
SwissProt
O43747

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV AKLLYMHMLG
61YPAHFGQLEC FKLIASQKFT DKRIGYLGAM LLLDERQDVH LLMTNCIKND LNHSTQFVQG
121LALCTLGCMG SSEMCRDLAG EVEKLLKTSN SYLRKKAALC AVHVIRKVPE LMEMFLPATK
181NLLNEKNHGV LHTSVVLLTE MCERSPDMLA HFRKNEKLVP QLVRILKNLI MSGYSPEHDV
241SGISDPFLQV RILRLLRILG RNDDDSSEAM NDILAQVATN TETSKNVGNA ILYETVLTIM
301DIKSESGLRV LAINILGRFL LNNDKNIRYV ALTSLLKTVQ TDHNAVQRHR STIVDCLKDL
361DVSIKRRAME LSFALVNGNN IRGMMKELLY FLDSCEPEFK ADCASGIFLA AEKYAPSKRW
421HIDTIMRVLT TAGSYVRDDA VPNLIQLITN SVEMHAYTVQ RLYKAILGDY SQQPLVQVAA
481WCIGEYGDLL VSGQCEEEEP IQVTEDEVLD ILESVLISNM STSVTRGYAL TAIMKLSTRF
541TCTVNRIKKV VSIYGSSIDV ELQQRAVEYN ALFKKYDHMR SALLERMPVM EKVTTNGPTE
601IVQTNGETEP APLETKPPPS GPQPTSQAND LLDLLGGNDI TPVIPTAPTS KPSSAGGELL
661DLLGDINLTG APAAAPAPAS VPQISQPPFL LDGLSSQPLF NDIAAGIPSI TAYSKNGLKI
721EFTFERSNTN PSVTVITIQA SNSTELDMTD FVFQAAVPKT FQLQLLSPSS SIVPAFNTGT
781ITQVIKVLNP QKQQLRMRIK LTYNHKGSAM QDLAEVNNFP PQSWQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

825 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.