Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AP1G1 / Adaptin Gamma 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118765-100
Catalog Number: LS-G118765
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human gamma Adaptin(AP1G1) (NM_001128), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ADTG | AP1G1 | AP-1 complex subunit gamma-1 | CLAPG1 | Gamma adaptin | Gamma1-adaptin
- Origin Species
- Human
- NCBI GenBank
- NM_001128
- SwissProt
- O43747
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV AKLLYMHMLG61YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH LLMTNCIKND LNHSTQFVQG121LALCTLGCMG SSEMCRDLAG EVEKLLKTSN SYLRKKAALC AVHVIRKVPE LMEMFLPATK181NLLNEKNHGV LHTSVVLLTE MCERSPDMLA HFRKNEKLVP QLVRILKNLI MSGYSPEHDV241SGISDPFLQV RILRLLRILG RNDDDSSEAM NDILAQVATN TETSKNVGNA ILYETVLTIM301DIKSESGLRV LAINILGRFL LNNDKNIRYV ALTSLLKTVQ TDHNAVQRHR STIVDCLKDL361DVSIKRRAME LSFALVNGNN IRGMMKELLY FLDSCEPEFK ADCASGIFLA AEKYAPSKRW421HIDTIMRVLT TAGSYVRDDA VPNLIQLITN SVEMHAYTVQ RLYKAILGDY SQQPLVQVAA481WCIGEYGDLL VSGQCEEEEP IQVTEDEVLD ILESVLISNM STSVTRGYAL TAIMKLSTRF541TCTVNRIKKV VSIYGSSIDV ELQQRAVEYN ALFKKYDHMR SALLERMPVM EKVTTNGPTE601IVQTNGETEP APLETKPPPS GPQPTSQAND LLDLLGGNDI TPVIPTAPTS KPSSAGGELL661DLLGDINLTG APAAAPAPAS VPQISQPPFL LDGLSSQPLF NDIAAGIPSI TAYSKNGLKI721EFTFERSNTN PSVTVITIQA SNSTELDMTD FVFQAAVPKT FQLQLLSPSS SIVPAFNTGT781ITQVIKVLNP QKQQLRMRIK LTYNHKGSAM QDLAEVNNFP PQSWQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
825 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.