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Human ANO7 / TMEM16G / NGEP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ANO7 / TMEM16G / NGEP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G88327-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Anoctamin 7(ANO7) (NM_001001891) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANO7 | Anoctamin 7 | Anoctamin-7 | D-TMPP | IPCA5 | NGEP | PCANAP5L | TMEM16G | New gene expressed in prostate | DTMPP | IPCA-5 | PCANAP5 | Transmembrane protein 16G
Origin Species
Human
NCBI GenBank
NM_001001891
SwissProt
Q6IWH7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRMAATAWAG LQGPPLPTLC PAVRTGLYCR DQAHAERWAM TSETSSGSHC ARSRMLRRRA
61QEEDSTVLID VSPPEAEKRG SYGSTAHASE PGGQQAAACR AGSPAKPRID FVLVWEEDLK
121LDRQQDSAAR DRTDMHRTWR ETFLDNLRAA GLCVDQQDVQ DGNTTVHYAL LSASWAVLCY
181YAEDLRLKLP LQELPNQASN WSAGLLAWLG IPNVLLEVVP DVPPEYYSCR FRVNKLPRFL
241GSDNQDTFFT STKRHQILFE ILAKTPYGHE KKNLLGIHQL LAEGVLSAAF PLHDGPFKTP
301PEGPQAPRLN QRQVLFQHWA RWGKWNKYQP LDHVRRYFGE KVALYFAWLG FYTGWLLPAA
361VVGTLVFLVG CFLVFSDIPT QELCGSKDSF EMCPLCLDCP FWLLSSACAL AQAGRLFDHG
421GTVFFSLFMA LWAVLLLEYW KRKSATLAYR WDCSDYEDTE ERPRPQFAAS APMTAPNPIT
481GEDEPYFPER SRARRMLAGS VVIVVMVAVV VMCLVSIILY RAIMAIVVSR SGNTLLAAWA
541SRIASLTGSV VNLVFILILS KIYVSLAHVL TRWEMHRTQT KFEDAFTLKV FIFQFVNFYS
601SPVYIAFFKG RFVGYPGNYH TLFGVRNEEC AAGGCLIELA QELLVIMVGK QVINNMQEVL
661IPKLKGWWQK FRLRSKKRKA GASAGASQGP WEDDYELVPC EGLFDEYLEM VLQFGFVTIF
721VAACPLAPLF ALLNNWVEIR LDARKFVCEY RRPVAERAQD IGIWFHILAG LTHLAVISNA
781FLLAFSSDFL PRAYYRWTRA HDLRGFLNFT LARAPSSFAA AHNRTCRYRA FRDDDGHYSQ
841TYWNLLAIRL AFVIVFEHVV FSVGRLLDLL VPDIPESVEI KVKREYYLAK QALAENEVLF
901GTNGTKDEQP EGSELSSHWT PFTVPKASQL QQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

932 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.