Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANO7 / TMEM16G / NGEP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122733-100
Catalog Number: LS-G122733
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Anoctamin 7(ANO7) (NM_001001891), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANO7 | Anoctamin 7 | Anoctamin-7 | D-TMPP | IPCA5 | NGEP | PCANAP5L | TMEM16G | New gene expressed in prostate | DTMPP | IPCA-5 | PCANAP5 | Transmembrane protein 16G
- Origin Species
- Human
- NCBI GenBank
- NM_001001891
- SwissProt
- Q6IWH7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 105.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRMAATAWAG LQGPPLPTLC PAVRTGLYCR DQAHAERWAM TSETSSGSHC ARSRMLRRRA61QEEDSTVLID VSPPEAEKRG SYGSTAHASE PGGQQAAACR AGSPAKPRID FVLVWEEDLK121LDRQQDSAAR DRTDMHRTWR ETFLDNLRAA GLCVDQQDVQ DGNTTVHYAL LSASWAVLCY181YAEDLRLKLP LQELPNQASN WSAGLLAWLG IPNVLLEVVP DVPPEYYSCR FRVNKLPRFL241GSDNQDTFFT STKRHQILFE ILAKTPYGHE KKNLLGIHQL LAEGVLSAAF PLHDGPFKTP301PEGPQAPRLN QRQVLFQHWA RWGKWNKYQP LDHVRRYFGE KVALYFAWLG FYTGWLLPAA361VVGTLVFLVG CFLVFSDIPT QELCGSKDSF EMCPLCLDCP FWLLSSACAL AQAGRLFDHG421GTVFFSLFMA LWAVLLLEYW KRKSATLAYR WDCSDYEDTE ERPRPQFAAS APMTAPNPIT481GEDEPYFPER SRARRMLAGS VVIVVMVAVV VMCLVSIILY RAIMAIVVSR SGNTLLAAWA541SRIASLTGSV VNLVFILILS KIYVSLAHVL TRWEMHRTQT KFEDAFTLKV FIFQFVNFYS601SPVYIAFFKG RFVGYPGNYH TLFGVRNEEC AAGGCLIELA QELLVIMVGK QVINNMQEVL661IPKLKGWWQK FRLRSKKRKA GASAGASQGP WEDDYELVPC EGLFDEYLEM VLQFGFVTIF721VAACPLAPLF ALLNNWVEIR LDARKFVCEY RRPVAERAQD IGIWFHILAG LTHLAVISNA781FLLAFSSDFL PRAYYRWTRA HDLRGFLNFT LARAPSSFAA AHNRTCRYRA FRDDDGHYSQ841TYWNLLAIRL AFVIVFEHVV FSVGRLLDLL VPDIPESVEI KVKREYYLAK QALAENEVLF901GTNGTKDEQP EGSELSSHWT PFTVPKASQL QQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
932 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.