LSBio
Human ANAPC7 / APC7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ANAPC7 / APC7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ANAPC7 antibodies →
$220.00each
Catalog Number: LS-G90301-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Apc7(ANAPC7) (NM_016238) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cyclosome subunit 7 | ANAPC7 | APC7
Origin Species
Human
NCBI GenBank
NM_016238
SwissProt
Q9UJX3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNVIDHVRDM AAAGLHSNVR LLSSLLLTMS NNNPELFSPP QKYQLLVYHA DSLFHDKEYR
61NAVSKYTMAL QQKKALSKTS KVRPSTGNSA STPQSQCLPS EIEVKYKMAE CYTMLKQDKD
121AIAILDGIPS RQRTPKINMM LANLYKKAGR ERPSVTSYKE VLRQCPLALD AILGLLSLSV
181KGAEVASMTM NVIQTVPNLD WLSVWIKAYA FVHTGDNSRA ISTICSLEKK SLLRDNVDLL
241GSLADLYFRA GDNKNSVLKF EQAQMLDLYL IKGMDVYGYL LAREGRLEDV ENLGCRLFNI
301SDQHAEPWVV SGCHSFYSKR YSRALYLGAK AIQLNSNSVQ ALLLKGAALR NMGRVQEAII
361HFREAIRLAP CRLDCYEGLI ECYLASNSIR EAMVMANNVY KTLGANAQTL TLLATVCLED
421PVTQEKAKTL LDKALTQRPD YIKAVVKKAE LLSREQKYED GIALLRNALA NQSDCVLHRI
481LGDFLVAVNE YQEAMDQYSI ALSLDPNDQK SLEGMQKMEK EESPTDATQE EDVDDMEGSG
541EEGDLEGSDS EAAQWADQEQ WFGMQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

565 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.