Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANAPC7 / APC7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112775-100
Catalog Number: LS-G112775
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Apc7(ANAPC7) (NM_016238), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cyclosome subunit 7 | ANAPC7 | APC7
- Origin Species
- Human
- NCBI GenBank
- NM_016238
- SwissProt
- Q9UJX3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNVIDHVRDM AAAGLHSNVR LLSSLLLTMS NNNPELFSPP QKYQLLVYHA DSLFHDKEYR61NAVSKYTMAL QQKKALSKTS KVRPSTGNSA STPQSQCLPS EIEVKYKMAE CYTMLKQDKD121AIAILDGIPS RQRTPKINMM LANLYKKAGR ERPSVTSYKE VLRQCPLALD AILGLLSLSV181KGAEVASMTM NVIQTVPNLD WLSVWIKAYA FVHTGDNSRA ISTICSLEKK SLLRDNVDLL241GSLADLYFRA GDNKNSVLKF EQAQMLDLYL IKGMDVYGYL LAREGRLEDV ENLGCRLFNI301SDQHAEPWVV SGCHSFYSKR YSRALYLGAK AIQLNSNSVQ ALLLKGAALR NMGRVQEAII361HFREAIRLAP CRLDCYEGLI ECYLASNSIR EAMVMANNVY KTLGANAQTL TLLATVCLED421PVTQEKAKTL LDKALTQRPD YIKAVVKKAE LLSREQKYED GIALLRNALA NQSDCVLHRI481LGDFLVAVNE YQEAMDQYSI ALSLDPNDQK SLEGMQKMEK EESPTDATQE EDVDDMEGSG541EEGDLEGSDS EAAQWADQEQ WFGMQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
565 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.