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Human AMPD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human AMPD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all AMPD3 antibodies →
$220.00each
Catalog Number: LS-G74486-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AMPD3 (NM_000480) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AMP deaminase 3 | AMP aminohydrolase | AMPD3 | Erythrocyte type AMP deaminase | AMP deaminase isoform E | Myoadenylate deaminase | Erythrocyte AMP deaminase
Origin Species
Human
NCBI GenBank
NM_000480
SwissProt
Q01432

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALSSEPAEM PRQFPKLNIS EVDEQVRLLA EKVFAKVLRE EDSKDALSLF TVPEDCPIGQ
61KEAKERELQK ELAEQKSVET AKRKKSFKMI RSQSLSLQMP PQQDWKGPPA ASPAMSPTTP
121VVTGATSLPT PAPYAMPEFQ RVTISGDYCA GITLEDYEQA AKSLAKALMI REKYARLAYH
181RFPRITSQYL GHPRADTAPP EEGLPDFHPP PLPQEDPYCL DDAPPNLDYL VHMQGGILFV
241YDNKKMLEHQ EPHSLPYPDL ETYTVDMSHI LALITDGPTK TYCHRRLNFL ESKFSLHEML
301NEMSEFKELK SNPHRDFYNV RKVDTHIHAA ACMNQKHLLR FIKHTYQTEP DRTVAEKRGR
361KITLRQVFDG LHMDPYDLTV DSLDVHAGRQ TFHRFDKFNS KYNPVGASEL RDLYLKTENY
421LGGEYFARMV KEVARELEES KYQYSEPRLS IYGRSPEEWP NLAYWFIQHK VYSPNMRWII
481QVPRIYDIFR SKKLLPNFGK MLENIFLPLF KATINPQDHR ELHLFLKYVT GFDSVDDESK
541HSDHMFSDKS PNPDVWTSEQ NPPYSYYLYY MYANIMVLNN LRRERGLSTF LFRPHCGEAG
601SITHLVSAFL TADNISHGLL LKKSPVLQYL YYLAQIPIAM SPLSNNSLFL EYSKNPLREF
661LHKGLHVSLS TDDPMQFHYT KEALMEEYAI AAQVWKLSTC DLCEIARNSV LQSGLSHQEK
721QKFLGQNYYK EGPEGNDIRK TNVAQIRMAF RYETLCNELS FLSDAMKSEE ITALTN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

776 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.