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AMPD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AMPD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120985-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AMPD3 (NM_001025390), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AMP deaminase 3 | AMP aminohydrolase | AMPD3 | Erythrocyte type AMP deaminase | AMP deaminase isoform E | Myoadenylate deaminase | Erythrocyte AMP deaminase
Origin Species
Human
NCBI GenBank
NM_000480
SwissProt
Q01432

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPGSAEMPR QFPKLNISEV DEQVRLLAEK VFAKVLREED SKDALSLFTV PEDCPIGQKE
61AKERELQKEL AEQKSVETAK RKKSFKMIRS QSLSLQMPPQ QDWKGPPAAS PAMSPTTPVV
121TGATSLPTPA PYAMPEFQRV TISGDYCAGI TLEDYEQAAK SLAKALMIRE KYARLAYHRF
181PRITSQYLGH PRADTAPPEE GLPDFHPPPL PQEDPYCLDD APPNLDYLVH MQGGILFVYD
241NKKMLEHQEP HSLPYPDLET YTVDMSHILA LITDGPTKTY CHRRLNFLES KFSLHEMLNE
301MSEFKELKSN PHRDFYNVRK VDTHIHAAAC MNQKHLLRFI KHTYQTEPDR TVAEKRGRKI
361TLRQVFDGLH MDPYDLTVDS LDVHAGRQTF HRFDKFNSKY NPVGASELRD LYLKTENYLG
421GEYFARMVKE VARELEESKY QYSEPRLSIY GRSPEEWPNL AYWFIQHKVY SPNMRWIIQV
481PRIYDIFRSK KLLPNFGKML ENIFLPLFKA TINPQDHREL HLFLKYVTGF DSVDDESKHS
541DHMFSDKSPN PDVWTSEQNP PYSYYLYYMY ANIMVLNNLR RERGLSTFLF RPHCGEAGSI
601THLVSAFLTA DNISHGLLLK KSPVLQYLYY LAQIPIAMSP LSNNSLFLEY SKNPLREFLH
661KGLHVSLSTD DPMQFHYTKE ALMEEYAIAA QVWKLSTCDL CEIARNSVLQ SGLSHQEKQK
721FLGQNYYKEG PEGNDIRKTN VAQIRMAFRY ETLCNELSFL SDAMKSEEIT ALTN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

774 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.