AMOT / Angiomotin Antibody
Catalog Number: LS-C779751-100
100 µL / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human AMOT / Angiomotin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89075-20
Catalog Number: LS-G89075
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Angiomotin(AMOT) (NM_133265) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Angiomotin | Angiomotin p130 isoform | Angiomotin p80 isoform | KIAA1071 | AMOT
- Origin Species
- Human
- NCBI GenBank
- AF286598
- SwissProt
- Q4VCS5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRAQPSSAS YQPVPADPFA IVSRAQQMVE ILSDENRNLR QELEGCYEKV ARLQKVETEI61QRVSEAYENL VKSSSKREAL EKAMRNKLEG EIRRMHDFNR DLRERLETAN KQLAEKEYEG121SEDTRKTISQ LFAKNKESQR EKEKLEAELA TARSTNEDQR RHIEIRDQAL SNAQAKVVKL181EEELKKKQVY VDKVEKMQQA LVQLQAACEK REQLEHRLRT RLERELESLR IQQRQGNCQP241TNVSEYNAAA LMELLREKEE RILALEADMT KWEQKYLEEN VMRHFALDAA ATVAAQRDTT301VISHSPNTSY DTALEARIQK EEEEILMANK RCLDMEGRIK TLHAQIIEKD AMIKVLQQRS361RKEPSKTEQL SCMRPAKSLM SISNAGSGLL SHSSTLTGSP IMEEKRDDKS WKGSLGILLG421GDYRAEYVPS TPSPVPPSTP LLSAHSKTGS RDCSTQTERG TESNKTAAVA PISVPAPVAA481AATAAAITAT AATITTTMVA AAPVAVAAAA APAAAAAPSP ATAAATAAAV SPAAAGQIPA541AASVASAAAV APSAAAAAAV QVAPAAPAPV PAPALVPVPA PAAAQASAPA QTQAPTSAPA601VAPTPAPTPT PAVAQAEVPP ASPATGPGPH RLSIPSLTCN PDKTDGPVFH SNTLERKTPI661QILGQEPDAE MVEYLITRTRPLEQKLISEEDLAANDILDYKDDDDKV
676 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
