Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AMOT / Angiomotin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107561-100
Catalog Number: LS-G107561
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Angiomotin(AMOT) (NM_133265), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Angiomotin | Angiomotin p130 isoform | Angiomotin p80 isoform | KIAA1071 | AMOT
- Origin Species
- Human
- NCBI GenBank
- AF286598
- SwissProt
- Q4VCS5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRAQPSSAS YQPVPADPFA IVSRAQQMVE ILSDENRNLR QELEGCYEKV ARLQKVETEI61QRVSEAYENL VKSSSKREAL EKAMRNKLEG EIRRMHDFNR DLRERLETAN KQLAEKEYEG121SEDTRKTISQ LFAKNKESQR EKEKLEAELA TARSTNEDQR RHIEIRDQAL SNAQAKVVKL181EEELKKKQVY VDKVEKMQQA LVQLQAACEK REQLEHRLRT RLERELESLR IQQRQGNCQP241TNVSEYNAAA LMELLREKEE RILALEADMT KWEQKYLEEN VMRHFALDAA ATVAAQRDTT301VISHSPNTSY DTALEARIQK EEEEILMANK RCLDMEGRIK TLHAQIIEKD AMIKVLQQRS361RKEPSKTEQL SCMRPAKSLM SISNAGSGLL SHSSTLTGSP IMEEKRDDKS WKGSLGILLG421GDYRAEYVPS TPSPVPPSTP LLSAHSKTGS RDCSTQTERG TESNKTAAVA PISVPAPVAA481AATAAAITAT AATITTTMVA AAPVAVAAAA APAAAAAPSP ATAAATAAAV SPAAAGQIPA541AASVASAAAV APSAAAAAAV QVAPAAPAPV PAPALVPVPA PAAAQASAPA QTQAPTSAPA601VAPTPAPTPT PAVAQAEVPP ASPATGPGPH RLSIPSLTCN PDKTDGPVFH SNTLERKTPI661QILGQEPDAE MVEYLITRTRPLEQKLISEEDLAANDILDYKDDDDKV
676 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.