LSBio
Human ALS2CR15 / ICA1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ALS2CR15 / ICA1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ICA1L antibodies →
$153.00each
Catalog Number: LS-G87237-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ALS2CR15(ICA1L) (NM_138468) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ALS2CR15 | ICA1L | Ica69-related protein | ALS2CR14
Origin Species
Human
NCBI GenBank
NM_138468
SwissProt
Q8NDH6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MDSFGQPRPE DNQSVVRRMQ KKYWKTKQVF IKATGKKEDE HLVASDAELD AKLEVFHSVQ
61ETCTELLKII EKYQLRLNVI SEEENELGLF LKFQAERDAT QAGKMMDATG KALCSSAKQR
121LALCTPLSRL KQEVATFSQR AVSDTLMTIN RMEQARTEYR GALLWMKDVS QELDPDTLKQ
181MEKFRKVQMQ VRNSKASFDK LKMDVCQKVD LLGASRCNML SHSLTTYQRT LLGFWKKTAR
241MMSQIHEACI GFHPYDFVAL KQLQDTPSKI SEDNKDEQIG GFLTEQLNKL VLSDEEASFE
301SEQANKDHNE KHSQMREFGA PQFSNSENVA KDLPVDSLEG EDFEKEFSFL NNLLSSGSSS
361TSEFTQECQT AFGSPSASLT SQEPSMGSEP LAHSSRFLPS QLFDLGFHVA GAFNNWVSQE
421ESELCLSHTD NQPVPSQSPK KLTRSPNNGN QDMSAWFNLF ADLDPLSNPD AIGHSDDELL
481NA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

482 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.