Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ALS2CR15 / ICA1L Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107302-100
Catalog Number: LS-G107302
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ALS2CR15(ICA1L) (NM_138468), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ALS2CR15 | ICA1L | Ica69-related protein | ALS2CR14
- Origin Species
- Human
- NCBI GenBank
- NM_138468
- SwissProt
- Q8NDH6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSFGQPRPE DNQSVVRRMQ KKYWKTKQVF IKATGKKEDE HLVASDAELD AKLEVFHSVQ61ETCTELLKII EKYQLRLNVI SEEENELGLF LKFQAERDAT QAGKMMDATG KALCSSAKQR121LALCTPLSRL KQEVATFSQR AVSDTLMTIN RMEQARTEYR GALLWMKDVS QELDPDTLKQ181MEKFRKVQMQ VRNSKASFDK LKMDVCQKVD LLGASRCNML SHSLTTYQRT LLGFWKKTAR241MMSQIHEACI GFHPYDFVAL KQLQDTPSKI SEDNKDEQIG GFLTEQLNKL VLSDEEASFE301SEQANKDHNE KHSQMREFGA PQFSNSENVA KDLPVDSLEG EDFEKEFSFL NNLLSSGSSS361TSEFTQECQT AFGSPSASLT SQEPSMGSEP LAHSSRFLPS QLFDLGFHVA GAFNNWVSQE421ESELCLSHTD NQPVPSQSPK KLTRSPNNGN QDMSAWFNLF ADLDPLSNPD AIGHSDDELL481NATRTRPLEQKLISEEDLAANDILDYKDDDDKV
482 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.