AGO3 / EIF2C3 Antibody
Catalog Number: LS-C348911-100
100 µL (1 mg/mL) / $339.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human AGO3 / EIF2C3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G88366-20
Catalog Number: LS-G88366
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AGO3(AGO3) (NM_177422) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Argonaute3 | Argonaute 3 | AGO3 | EIF-2C 3 | HAgo3 | EIF2C 3 | EIF2C3 | Protein argonaute-3
- Origin Species
- Human
- NCBI GenBank
- NM_024852
- SwissProt
- Q9H9G7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCEVLDIHNI DEQPRPLTDS HRVKFTKEIK GLKVEVTHCG TMRRKYRVCN VTRRPASHQT61FPLQLENGQT VERTVAQYFR EKYTLQLKYP HLPCLQVGQE QKHTYLPLEV CNIVAGQRCI121KKLTDNQTST MIKATARSAP DRQEEISRLV RSANYETDPF VQEFQFKVRD EMAHVTGRVL181PAPMLQYGGR NRTVATPSHG VWDMRGKQFH TGVEIKMWAI ACFATQRQCR EEILKGFTDQ241LRKISKDAGM PIQGQPCFCK YAQGADSVEP MFRHLKNTYS GLQLIIVILP GKTPVYAEVK301RVGDTLLGMA TQCVQVKNVI KTSPQTLSNL CLKINVKLGG INNILVPHQR PSVFQQPVIF361LGADVTHPPA GDGKKPSIAA VVGSMDAHPS RYCATVRVQR PRQEIIQDLA SMVRELLIQF421YKSTRFKPTR IIFYRDGVSE GQFRQVLYYE LLAIREACIS LEKDYQPGIT YIVVQKRHHT481RLFCADRTER VGRSGNIPAG TTVDTDITHP YEFDFYLCSH AGIQGTSRPS HYHVLWDDNC541FTADELQLLT YQLCHTYVRC TRSVSIPAPA YYAHLVAFRA RYHLVDKEHD SAEGSHVSGQ601SNGRDPQALA KAVQIHQDTL RTMYFASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
626 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
