Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AGO3 / EIF2C3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104901-100
Catalog Number: LS-G104901
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGO3(AGO3) (NM_177422), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Argonaute3 | Argonaute 3 | AGO3 | EIF-2C 3 | HAgo3 | EIF2C 3 | EIF2C3 | Protein argonaute-3
- Origin Species
- Human
- NCBI GenBank
- NM_024852
- SwissProt
- Q9H9G7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCEVLDIHNI DEQPRPLTDS HRVKFTKEIK GLKVEVTHCG TMRRKYRVCN VTRRPASHQT61FPLQLENGQT VERTVAQYFR EKYTLQLKYP HLPCLQVGQE QKHTYLPLEV CNIVAGQRCI121KKLTDNQTST MIKATARSAP DRQEEISRLV RSANYETDPF VQEFQFKVRD EMAHVTGRVL181PAPMLQYGGR NRTVATPSHG VWDMRGKQFH TGVEIKMWAI ACFATQRQCR EEILKGFTDQ241LRKISKDAGM PIQGQPCFCK YAQGADSVEP MFRHLKNTYS GLQLIIVILP GKTPVYAEVK301RVGDTLLGMA TQCVQVKNVI KTSPQTLSNL CLKINVKLGG INNILVPHQR PSVFQQPVIF361LGADVTHPPA GDGKKPSIAA VVGSMDAHPS RYCATVRVQR PRQEIIQDLA SMVRELLIQF421YKSTRFKPTR IIFYRDGVSE GQFRQVLYYE LLAIREACIS LEKDYQPGIT YIVVQKRHHT481RLFCADRTER VGRSGNIPAG TTVDTDITHP YEFDFYLCSH AGIQGTSRPS HYHVLWDDNC541FTADELQLLT YQLCHTYVRC TRSVSIPAPA YYAHLVAFRA RYHLVDKEHD SAEGSHVSGQ601SNGRDPQALA KAVQIHQDTL RTMYFASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
626 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.