ADGRD1 / GPR133 Antibody
Catalog Number: LS-C809209
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ADGRD1 / GPR133 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G92235-20
Catalog Number: LS-G92235
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GPR133(ADGRD1) (NM_198827) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 133 | GPR133 | PGR25
- Origin Species
- Human
- NCBI GenBank
- AY278561
- SwissProt
- Q6QNK2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 96.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKLLRLCCW YSWLLLFYYN FQVRGVYSRS QDHPGFQVLA SASHYWPLEN VDGIHELQDT61TGDIVEGKVN KGIYLKEEKG VTLLYYGRYN SSCISKPEQC GPEGVTFSFF WKTQGEQSRP121IPSAYGGQVI SNGFKVCSSG GRGSVELYTR DNSMTWEASF SPPGPYWTHV LFTWKSKEGL181KVYVNGTLST SDPSGKVSRD YGESNVNLVI GSEQDQAKCY ENGAFDEFII WERALTPDEI241AMYFTAAIGK HALLSSTLPS LFMTSTASPV MPTDAYHPII TNLTEERKTF QSPGVILSYL301QNVSLSLPSK SLSEQTALNL TKTFLKAVGE ILLLPGWIAL SEDSAVVLSL IDTIDTVMGH361VSSNLHGSTP QVTVEGSSAM AEFSVAKILP KTVNSSHYRF PAHGQSFIQI PHEAFHRHAW421STVVGLLYHS MHYYLNNIWP AHTKIAEAMH HQDCLLFATS HLISLEVSPP PTLSQNLSGS481PLITVHLKHR LTRKQHSEAT NSSNRVFVYC AFLDFSSGEG VWSNHGCALT RGNLTYSVCR541CTHLTNFAIL MQVVPLELAR GHQVALSSIS YVGCSLSVLC LVATLVTFAV LSSVSTIRNQ601RYHIHANLSF AVLVAQVLLL ISFRLEPGTT PCQVMAVLLH YFFLSAFAWM LVEGLHLYSM661VIKVFGSEDS KHRYYYGMGW GFPLLICIIS LSFAMDSYGT SNNCWLSLAS GAIWAFVAPA721LFVIVVNIGI LIAVTRVISQ ISADNYKIHG DPSAFKLTAK AVAVLLPILG TSWVFGVLAV781NGCAVVFQYM FATLNSLQGL FIFLFHCLLN SEVRAAFKHK TKVWSLTSSS ARTSNAKPFH841SDLMNGTRPG MASTKLSPWD KSSHSAHRVD LSAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
874 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
