Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRD1 / GPR133 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102574-100
Catalog Number: LS-G102574
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR133(ADGRD1) (NM_198827), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 133 | GPR133 | PGR25
- Origin Species
- Human
- NCBI GenBank
- AY278561
- SwissProt
- Q6QNK2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 96.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKLLRLCCW YSWLLLFYYN FQVRGVYSRS QDHPGFQVLA SASHYWPLEN VDGIHELQDT61TGDIVEGKVN KGIYLKEEKG VTLLYYGRYN SSCISKPEQC GPEGVTFSFF WKTQGEQSRP121IPSAYGGQVI SNGFKVCSSG GRGSVELYTR DNSMTWEASF SPPGPYWTHV LFTWKSKEGL181KVYVNGTLST SDPSGKVSRD YGESNVNLVI GSEQDQAKCY ENGAFDEFII WERALTPDEI241AMYFTAAIGK HALLSSTLPS LFMTSTASPV MPTDAYHPII TNLTEERKTF QSPGVILSYL301QNVSLSLPSK SLSEQTALNL TKTFLKAVGE ILLLPGWIAL SEDSAVVLSL IDTIDTVMGH361VSSNLHGSTP QVTVEGSSAM AEFSVAKILP KTVNSSHYRF PAHGQSFIQI PHEAFHRHAW421STVVGLLYHS MHYYLNNIWP AHTKIAEAMH HQDCLLFATS HLISLEVSPP PTLSQNLSGS481PLITVHLKHR LTRKQHSEAT NSSNRVFVYC AFLDFSSGEG VWSNHGCALT RGNLTYSVCR541CTHLTNFAIL MQVVPLELAR GHQVALSSIS YVGCSLSVLC LVATLVTFAV LSSVSTIRNQ601RYHIHANLSF AVLVAQVLLL ISFRLEPGTT PCQVMAVLLH YFFLSAFAWM LVEGLHLYSM661VIKVFGSEDS KHRYYYGMGW GFPLLICIIS LSFAMDSYGT SNNCWLSLAS GAIWAFVAPA721LFVIVVNIGI LIAVTRVISQ ISADNYKIHG DPSAFKLTAK AVAVLLPILG TSWVFGVLAV781NGCAVVFQYM FATLNSLQGL FIFLFHCLLN SEVRAAFKHK TKVWSLTSSS ARTSNAKPFH841SDLMNGTRPG MASTKLSPWD KSSHSAHRVD LSAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
874 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.