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Human ADAP / FYB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ADAP / FYB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FYB antibodies →
$220.00each
Catalog Number: LS-G80021-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FYB (NM_001465) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAP | FYN-binding protein | FYN-T-binding protein | FYB-120/130 | FYN binding protein | p120/p130 | PRO0823 | SLAP130 | FYB | SLAP-130
Origin Species
Human
NCBI GenBank
NM_001465
SwissProt
O15117

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKYNTGGNP TEDVSVNSRP FRVTGPNSSS GIQARKNLFN NQGNASPPAG PSNVPKFGSP
61KPPVAVKPSS EEKPDKEPKP PFLKPTGAGQ RFGTPASLTT RDPEAKVGFL KPVGPKPINL
121PKEDSKPTFP WPPGNKPSLH SVNQDHDLKP LGPKSGPTPP TSENEQKQAF PKLTGVKGKF
181MSASQDLEPK PLFPKPAFGQ KPPLSTENSH EDESPMKNVS SSKGSPAPLG VRSKSGPLKP
241AREDSENKDH AGEISSLPFP GVVLKPAASR GGPGLSKNGE EKKEDRKIDA AKNTFQSKIN
301QEELASGTPP ARFPKAPSKL TVGGPWGQSQ EKEKGDKNSA TPKQKPLPPL FTLGPPPPKP
361NRPPNVDLTK FHKTSSGNST SKGQTSYSTT SLPPPPPSHP ASQPPLPASH PSQPPVPSLP
421PRNIKPPFDL KSPVNEDNQD GVTHSDGAGN LDEEQDSEGE TYEDIEASKE REKKREKEEK
481KRLELEKKEQ KEKEKKEQEI KKKFKLTGPI QVIHLAKACC DVKGGKNELS FKQGEQIEII
541RITDNPEGKW LGRTARGSYG YIKTTAVEID YDSLKLKKDS LGAPSRPIED DQEVYDDVAE
601QDDISSHSQS GSGGIFPPPP DDDIYDGIEE EDADDGSTLQ VQEKSNTWSW GILKMLKGKD
661DRKKSIREKP KVSDSDNNEG SSFPAPPKQL DMGDEVYDDV DTSDFPVSSA EMSQGTNFGK
721AKTEEKDLKK LKKQEKEEKD FRKKFKYDGE IRVLYSTKVT TSITSKKWGT RDLQVKPGES
781LEVIQTTDDT KVLCRNEEGK YGYVLRSYLA DNDGEIYDDI ADGCIYDND
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

829 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.